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Screening and in silico characterization of prophages in Helicobacter pylori clinical strains

2025-03Artigo científico Acesso aberto
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dc.contributor.authorFerreira, Rute
dc.contributor.authorPinto, Graça
dc.contributor.authorPresa, Eva
dc.contributor.authorOleastro, Mónica
dc.contributor.authorSilva, Catarina
dc.contributor.authorVieira, Luís
dc.contributor.authorSousa, Cláudia
dc.contributor.authorPires, Diana P.
dc.contributor.authorFigueiredo, Ceu
dc.contributor.authorMelo, Luís D.R.
dc.contributor.institutionCentre for Toxicogenomics and Human Health (ToxOmics)
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.pblElsevier Science B.V., Inc
dc.date.accessioned2024-10-21T23:23:10Z
dc.date.available2024-10-21T23:23:10Z
dc.date.issued2025-03
dc.descriptionPublisher Copyright: © The Authors
dc.description.abstractThe increase of antibiotic resistance calls for alternatives to control Helicobacter pylori, a Gram-negative bacterium associated with various gastric diseases. Bacteriophages (phages) can be highly effective in the treatment of pathogenic bacteria. Here, we developed a method to identify prophages in H. pylori genomes aiming at their future use in therapy. A polymerase chain reaction (PCR)-based technique tested five primer pairs on 74 clinical H. pylori strains. After the PCR screening, 14 strains most likely to carry prophages were fully sequenced. After that, a more holistic approach was taken by studying the complete genome of the strains. This study allowed us to identify 12 intact prophage sequences, which were then characterized concerning their morphology, virulence, and antibiotic-resistance genes. To understand the variability of prophages, a phylogenetic analysis using the sequences of all H. pylori phages reported to date was performed. Overall, we increased the efficiency of identifying complete prophages to 54.1 %. Genes with homology to potential virulence factors were identified in some new prophages. Phylogenetic analysis revealed a close relationship among H. pylori-phages, although there are phages with different geographical origins. This study provides a deeper understanding of H. pylori-phages, providing valuable insights into their potential use in therapy.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent4359354
dc.identifier.doi10.1016/j.micinf.2024.105429
dc.identifier.issn1286-4579
dc.identifier.otherPURE: 101499408
dc.identifier.otherPURE UUID: 94064394-483f-4564-9ada-52b873765160
dc.identifier.otherScopus: 85205961788
dc.identifier.otherPubMed: 39368610
dc.identifier.urihttp://hdl.handle.net/10362/173843
dc.identifier.urlhttps://www.scopus.com/pages/publications/85205961788
dc.language.isoeng
dc.peerreviewedyes
dc.subjectAntimicrobial resistance genes
dc.subjectHelicobacter pylori
dc.subjectPCR
dc.subjectProphages
dc.subjectVirulence factors
dc.subjectMicrobiology
dc.subjectImmunology
dc.subjectInfectious Diseases
dc.subjectSDG 3 - Good Health and Well-being
dc.titleScreening and in silico characterization of prophages in Helicobacter pylori clinical strainsen
dc.typejournal article
degois.publication.issue3
degois.publication.titleMicrobes And Infection
degois.publication.volume27
dspace.entity.typePublication
rcaap.rightsopenAccess

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