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Synthesis of Silver Nanoparticle Employing Corn Cob Xylan as a Reducing Agent with Anti-Trypanosoma cruzi Activity

2020Artigo científico Acesso aberto
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dc.contributor.authorBrito, Talita Katiane
dc.contributor.authorSilva Viana, Rony Lucas
dc.contributor.authorGonçalves Moreno, Cláudia Jassica
dc.contributor.authorda Silva Barbosa, Jefferson
dc.contributor.authorde Sousa, Francimar Lopes
dc.contributor.authorCampos de Medeiros, Mayara Jane
dc.contributor.authorMelo-Silveira, Raniere Fagundes
dc.contributor.authorAlmeida-Lima, Jailma
dc.contributor.authorde Lima Pontes, Daniel
dc.contributor.authorSousa Silva, Marcelo
dc.contributor.authorRocha, Hugo Alexandre Oliveira
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.institutionVector borne diseases and pathogens (VBD)
dc.contributor.pblDove Medical Press
dc.date.accessioned2025-03-17T21:27:55Z
dc.date.available2025-03-17T21:27:55Z
dc.date.issued2020
dc.descriptionPublisher Copyright: © 2020 Brito et al. Acknowledgments The authors wish to thank Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq, Coordenação de Aperfeiçoamento Pessoal de Nível Superior-CAPES and Ministério de Ciência, Tecnologia, Informação e Comércio – MCTIC for the financial support. Hugo Rocha is CNPq fellowship honored researchers. To the Department of Biochemistry of the UFRN for providing the flow cytometer where the experiments presented in this paper were performed.
dc.description.abstractBackground: Chagas disease, also known as American Trypanosomiasis, is caused by the protozoan Trypanosoma cruzi. It is occurring in Americas, including USA and Canada, and Europe and its current treatment involves the use of two drugs as follows: benznidazole (BNZ) and nifurtimox, which present high toxicity and low efficacy during the chronic phase of the disease, thus promoting the search for more effective therapeutic alternatives. Amongst them xylan, a bioactive polysaccharide, extracted from corn cob. Methods: Ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy (FITR), Raman spectroscopy, energy-dispersive X-ray spectroscopy (EDS), scanning electron microscopy, atomic force microscopy, plasma optical emission spectroscopy (ICP-OES), dynamic light scattering (DLS) have been used to characterize the silver-xylan nanoparticles (NX). Their cytotoxicity was evaluated with 3-bromo(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) test. MTT and flow cytometry were used to ascertain the anti-Trypanosoma cruzi activity. Results: UV-Vis spectroscopy gave plasmon resonance ranging between 400 and 450 nm while FITC and Raman spectroscopy proved nano interface functionalized with xylan. ICP-OES data showed NX with xylan (81%) and silver (19%). EDS showed NX consisting of carbon (59.4%), oxygen (26.2%) and silver (4.8%) main elements. Spherical NX of 55 nm average size has been depicted with SEM and AFM, while DLS showed 102 ± 1.7 nm NX. The NX displayed negligible cytotoxicity (2000 µg/mL). NX (100 µg/mL) was more effective, regardless of experiment time, in affecting the ability of parasites to reduce MTT than BZN (100 µg/mL). In addition, NX (100 µg/mL) induced death of 95% of parasites by necrosis. Conclusion: This is the first time silver nanoparticles are presented as an anti-Trypanosoma cruzi agent and the data point to the potential application of NX to new preclinical studies in vitro and in vivo.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent15
dc.format.extent6887522
dc.identifier.doi10.2147/IJN.S216386
dc.identifier.issn1178-2013
dc.identifier.otherPURE: 113041774
dc.identifier.otherPURE UUID: 2a66c48b-e9bf-4b5b-95e4-0dcb94414ae7
dc.identifier.otherScopus: 85080084626
dc.identifier.otherWOS: 000513132700001
dc.identifier.otherPubMed: 32103950
dc.identifier.otherPubMedCentral: PMC7026134
dc.identifier.urihttp://hdl.handle.net/10362/180784
dc.identifier.urlhttps://www.scopus.com/pages/publications/85080084626
dc.language.isoeng
dc.peerreviewedyes
dc.subjectbenznidazole
dc.subjectsilver nanoparticles
dc.subjectTrypanosoma cruzi
dc.subjectxylan
dc.subjectBiophysics
dc.subjectBioengineering
dc.subjectBiomaterials
dc.subjectPharmaceutical Science
dc.subjectDrug Discovery
dc.subjectOrganic Chemistry
dc.subjectSDG 3 - Good Health and Well-being
dc.titleSynthesis of Silver Nanoparticle Employing Corn Cob Xylan as a Reducing Agent with Anti-Trypanosoma cruzi Activityen
dc.typejournal article
degois.publication.firstPage965
degois.publication.lastPage979
degois.publication.titleInternational journal of nanomedicine
degois.publication.volume15
dspace.entity.typePublication
rcaap.rightsopenAccess

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