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Molecular signatures of xenograft colorectal cancer in mice treated with topotecan

2025-06Artigo científico Acesso aberto
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dc.contributor.authorHagyousif, Yousra A.
dc.contributor.authorZenati, Ruba A.
dc.contributor.authorSoares, Nelson C.
dc.contributor.authorAl-Hroub, Hamza M.
dc.contributor.authorKhan, Farman Matloob
dc.contributor.authorQaisar, Rizwan
dc.contributor.authorHamoudi, Rifat
dc.contributor.authorEl-Awady, Raafat
dc.contributor.authorAbuhelwa, Ahmad Y.
dc.contributor.authorRamadan, Wafaa
dc.contributor.authorEl-Huneidi, Waseem
dc.contributor.authorAbu-Gharbieh, Eman
dc.contributor.authorAlzoubi, Karem H.
dc.contributor.authorBustanji, Yasser
dc.contributor.authorSemreen, Mohammad H.
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.institutionComprehensive Health Research Centre (CHRC) - pólo NMS
dc.contributor.pblElsevier
dc.date.accessioned2025-05-27T21:25:09Z
dc.date.available2025-05-27T21:25:09Z
dc.date.issued2025-06
dc.descriptionFunding Information: The authors would like to acknowledge The University of Sharjah financial support. This study was financially supported by the University of Sharjah (competitive grant number 2201110155 and targeted grant number 24011101105 ). Publisher Copyright: © 2025 The Authors
dc.description.abstractBackground: Colorectal cancer (CRC) is one of the most common cancers worldwide, yet it continues to have a low survival rate, largely due to the lack of effective treatments. Metabolomics offers new insight into disease diagnosis and biomarkers discovery. The aim of the study is to identify serum biomarkers in a CRC xenograft mouse model treated with topotecan using advanced metabolomics techniques to enhance our understanding and management of the disease. Methods: The therapeutic potentials of the anticancer drug topotecan on metabolomic alterations in CRC were explored using the UHPLC-ESI-QTOF-MS platform. A comprehensive metabolomic analysis was conducted to compare four different animal groups: HCT-116 CRC xenograft mice treated with topotecan (treated group), vehicle-control HCT-116 xenograft mice (untreated CRC xenograft mice), positive controls (healthy mice injected with topotecan), and negative controls (healthy mice). Results: The study identified 53 altered metabolites across all four groups (p-value < 0.05). Independent T-test revealed that 15 metabolites were statistically significant among vehicle controls and negative controls. Additionally, 20 metabolites showed significant differences between the potential responders to topotecan and the vehicle controls. Moreover, only one metabolite was statistically significant between the positive and negative controls. Conclusion: The findings provide a detailed characterization of metabolic alterations associated with topotecan treatment in CRC. These insights contribute to a better understanding of the drug's mechanism of action, which may help predict CRC patients’ response to topotecan and guide the development of personalized therapeutic strategies.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent5610541
dc.identifier.doi10.1016/j.toxrep.2025.102045
dc.identifier.issn2214-7500
dc.identifier.otherPURE: 116782092
dc.identifier.otherPURE UUID: 842746a5-d673-4a4d-8738-9584e9dfca3a
dc.identifier.otherScopus: 105005290441
dc.identifier.urihttp://hdl.handle.net/10362/183523
dc.identifier.urlhttps://www.scopus.com/pages/publications/105005290441
dc.language.isoeng
dc.peerreviewedyes
dc.subjectColorectal cancer
dc.subjectLC-MS/MS-QTOF
dc.subjectMetabolomics
dc.subjectTopotecan
dc.subjectToxicology
dc.subjectHealth, Toxicology and Mutagenesis
dc.subjectSDG 3 - Good Health and Well-being
dc.titleMolecular signatures of xenograft colorectal cancer in mice treated with topotecanen
dc.title.subtitleA mass spectrometry-based studyen
dc.typejournal article
degois.publication.titleToxicology Reports
degois.publication.volume14
dspace.entity.typePublication
rcaap.rightsopenAccess

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