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Six feet under microbiota

2022-05Artigo científico Acesso aberto
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dc.contributor.authorViegas, Carla
dc.contributor.authorCervantes, Renata
dc.contributor.authorDias, Marta
dc.contributor.authorGomes, Bianca
dc.contributor.authorPena, Pedro
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorTwaruzek, Magdalena
dc.contributor.authorKosicki, Robert
dc.contributor.authorSoszczyliska, Ewelina
dc.contributor.authorViegas, Susana
dc.contributor.authorCaetano, Liliana Aranha
dc.contributor.institutionComprehensive Health Research Centre (CHRC) - Pólo ENSP
dc.contributor.institutionCentro de Investigação em Saúde Pública (CISP/PHRC)
dc.contributor.institutionEscola Nacional de Saúde Pública (ENSP)
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2022-06-08T22:26:01Z
dc.date.available2022-06-08T22:26:01Z
dc.date.issued2022-05
dc.description.abstractCemeteries are potential environmental reservoirs of pathogenic microorganisms from organic matter decomposition. This study aimed to characterize the microbial contamination in three cemeteries, and more specifically in grave diggers’ facilities. One active sampling method (impingement method) and several passive sampling methods (swabs, settled dust, settled dust filters and electrostatic dust cloths—EDC) were employed. The molecular detection of Aspergillus sections and SARS-CoV-2, as well as mycotoxin analysis, screening of azole resistance, and cytotoxicity measurement were also conducted. Total bacteria contamination was 80 CFU.m−2 in settled dust samples, reached 849 CFU.m−2 in EDC and 20,000 CFU.m−2 in swabs, and ranged from 5000 to 10,000 CFU.m−2 in filters. Gram-negative bacteria (VRBA) were only observed in in settled dust samples (2.00x105 CFU.m−2). Regarding Aspergillus sp., the highest counts were obtained in DG18 (18.38%) and it was not observed in azole-supplemented SDA media. SARS-CoV-2 and the targeted Aspergillus sections were not detected. Mycophenolic acid was detected in one settled dust sample. Cytotoxic effects were observed for 94.4% filters and 5.6% EDC in A549 lung epithelial cells, and for 50.0% filters and 5.6% EDC in HepG2 cells. Future studies are needed in this occupational setting to implement more focused risk management measures.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent465180
dc.identifier.doi10.3390/toxins14050348
dc.identifier.issn2072-6651
dc.identifier.otherPURE: 44518975
dc.identifier.otherPURE UUID: 52af04c9-f7a0-49b4-9730-686b62460000
dc.identifier.otherWOS: 000801625000001
dc.identifier.otherPubMed: 35622594
dc.identifier.otherPubMedCentral: PMC9146229
dc.identifier.urihttp://hdl.handle.net/10362/139684
dc.language.isoeng
dc.peerreviewedyes
dc.subjectcemeteries
dc.subjectoccupational health
dc.subjectAspergillus
dc.subjectSARS-CoV-2
dc.subjectazole resistance
dc.subjectmycotoxins
dc.subjectcytotoxicity
dc.titleSix feet under microbiotaen
dc.title.subtitlemicrobiologic contamination and toxicity profile in three urban cemeteries from Lisbon, Portugalen
dc.typejournal article
degois.publication.issue5
degois.publication.titleToxins
degois.publication.volume14
dspace.entity.typePublication
rcaap.rightsopenAccess

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