Characterization of the Thermostable Biosurfactant Produced by Burkholderia thailandensis DSM 13276

Gil, Cátia V.; Rebocho, Ana Teresa; Esmail, Asiyah; Sevrin, Chantal; Grandfils, Christian; Torres, Cristiana A.V.; Reis, Maria A.M.; Freitas, Filomena

http://hdl.handle.net/10362/145493

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Autores

Torres, Cristiana A.V.

Reis, Maria A.M.

Freitas, Filomena

Resumo(s)

Biosurfactants synthesized by microorganisms represent safe and sustainable alternatives to the use of synthetic surfactants, due to their lower toxicity, better biodegradability and biocompatibility, and their production from low-cost feedstocks. In line with this, the present study describes the physical, chemical, and functional characterization of the biopolymer secreted by the bacterium Burkholderia thailandensis DSM 13276, envisaging its validation as a biosurfactant. The biopolymer was found to be a glycolipopeptide with carbohydrate and protein contents of 33.1 ± 6.4% and 23.0 ± 3.2%, respectively. Galactose, glucose, rhamnose, mannose, and glucuronic acid were detected in the carbohydrate moiety at a relative molar ratio of 4:3:2:2:1. It is a high-molecular-weight biopolymer (1.0×107Da) with low polydispersity (1.66), and forms aqueous solutions with shear-thinning behavior, which remained after autoclaving. The biopolymer has demonstrated a good emulsionstabilizing capacity towards different hydrophobic compounds, namely, benzene, almond oil, and sunflower oil. The emulsions prepared with the biosurfactant, as well as with its autoclaved solution, displayed high emulsification activity (>90% and ~50%, respectively). Moreover, the almond and sunflower oil emulsions stabilized with the biosurfactant were stable for up to 4 weeks, which further supports the potential of this novel biopolymer for utilization as a natural bioemulsifier.

Descrição

project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB; National Natural Science Foundation of China (grant numbers: Institute of Microbiology, Chinese Academy of Sciences: 31961133016; Beijing Institute of Technology: 31961133015; Shandong University: 31961133014). C.V.G., A.E. and A.T.R. acknowledge FCT I.P. for the Ph.D. grants 2021.05798.BD, 2021.05014.BD and 2020.06470.BD, respectively. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.