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Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration

2014-06-15Artigo científico Acesso aberto
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dc.contributor.authorCasalou, Cristina
dc.contributor.authorSeixas, Cecília
dc.contributor.authorPortelinha, Ana
dc.contributor.authorPintado, Petra
dc.contributor.authorBarros, Mafalda
dc.contributor.authorRamalho, José da Silva
dc.contributor.authorLopes, Susana Santos
dc.contributor.authorBarral, Duarte
dc.contributor.authorC. Barral, Duarte
dc.contributor.institutionCentro de Estudos de Doenças Crónicas (CEDOC)
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.pblCompany of Biologists Ltd
dc.date.accessioned2017-07-13T22:00:47Z
dc.date.available2017-07-13T22:00:47Z
dc.date.issued2014-06-15
dc.descriptionThis work was supported by a Marie Curie International Reintegration Grant [grant number Marie Curie_PIRG05-GA-2009-247726 to D.C.B.]; by postdoctoral fellowships from Fundacao para a Ciencia e Tecnologia (FCT) [grant numbers SFRH/BPD/78561/2011 to C.C., SFRH/BPD/32323/2006 to C.S.]; a PhD fellowship from FCT [grant number SFRH/BD/68502/2010 to A.P.]; and a FCT grant [grant number PTDC/SAU-OBD/103981/2008 to S.L.].
dc.description.abstractThe Arf-like protein Arl13b has been implicated in ciliogenesis and Sonic hedgehog signaling. Furthermore, we have previously shown that it regulates endocytic recycling traffic and interacts with actin. Herein, we report that the non-muscle myosin heavy chain IIA, also known as Myh9, is an Arl13b effector. Moreover, we found that both proteins localized to circular dorsal ruffles (CDRs) induced by platelet-derived growth factor stimulation and are required for their formation. CDRs are ring-shaped actin-dependent structures formed on the dorsal cell surface and are involved in diverse processes, such as macropinocytosis, integrin recycling, internalization of receptor tyrosine kinases and cell migration. We found that Arl13b or Myh9 silencing impaired cell migration, suggesting that Arl13b is required for this function through the interaction with Myh9. Moreover, Arl13b silencing impaired neural crest cell migration in zebrafish embryos. Furthermore, we showed that Arl13b is required for the formation of CDRs in migrating cells. Thus, our results indicate a new role for Arl13b in actin cytoskeleton remodeling through the interaction with Myh9, by driving the formation of CDRs necessary for cell migration.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent13
dc.format.extent10367070
dc.identifier.doi10.1242/jcs.143446
dc.identifier.issn0021-9533
dc.identifier.otherPURE: 390083
dc.identifier.otherPURE UUID: 52d27211-cc37-467d-aa97-d34912b6762d
dc.identifier.otherresearchoutputwizard: 45682
dc.identifier.otherPubMed: 24777479
dc.identifier.otherWOS: 000338443500011
dc.identifier.otherScopus: 84902436592
dc.identifier.otherORCID: /0000-0001-8867-2407/work/123561624
dc.identifier.urihttp://hdl.handle.net/10362/21937
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F32323%2F2006/PT
dc.relationTRAFFICKING PATHWAYS INVOVED IN THE ASSEMBLY AND MAINTENANCE OF PRIMARY CILIA.
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F68502%2F2010/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/103981/PT
dc.subjectActin cytoskeleton
dc.subjectLOCALIZATION
dc.subjectArl13b
dc.subjectPROTEINS
dc.subjectWound healing
dc.subjectMACROPINOCYTOSIS
dc.subjectCircular dorsal ruffle
dc.subjectSmall G protein
dc.subjectZEBRAFISH
dc.subjectPlatelet-derived growth factor signaling
dc.subjectPRIMARY CILIA
dc.subjectMyosin heavy chain IIA
dc.subjectACTIN
dc.subjectMyh9
dc.subjectLEAD
dc.subjectJOUBERT-SYNDROME
dc.subjectRECEPTOR
dc.subjectROLES
dc.subjectArl13b
dc.subjectMyosin heavy chain IIA
dc.subjectMyh9
dc.subjectCircular dorsal ruffle
dc.subjectSmall G protein
dc.subjectActin cytoskeleton
dc.subjectPlatelet-derived growth factor signaling
dc.subjectWound healing
dc.titleArl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migrationen
dc.typejournal article
degois.publication.firstPage2709
degois.publication.issue12
degois.publication.lastPage2722
degois.publication.titleJournal of Cell Science
degois.publication.volume127
dspace.entity.typePublication
oaire.awardNumberSFRH/BPD/32323/2006
oaire.awardNumberSFRH/BPD/78561/2011
oaire.awardNumberSFRH/BD/68502/2010
oaire.awardNumberPTDC/SAU-OBD/103981/2008
oaire.awardTitleTRAFFICKING PATHWAYS INVOVED IN THE ASSEMBLY AND MAINTENANCE OF PRIMARY CILIA.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F32323%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F78561%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F68502%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-OBD%2F103981%2F2008/PT
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
oaire.fundingStream3599-PPCDT
person.familyNameBarral
person.givenNameDuarte
person.identifier.ciencia-id8810-DEBC-025E
person.identifier.orcid0000-0001-8867-2407
person.identifier.ridE-7548-2013
person.identifier.scopus-author-id6602513159
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
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