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Comparative proteomic analysis of nodulated and non-nodulated casuarina glauca sieb. Ex spreng. grown under salinity conditions using sequential window acquisition of all theoretical mass spectra (SWATH-MS)

2020-01-01Artigo científico Acesso aberto
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dc.contributor.authorGraça, Inês
dc.contributor.authorMendes, Vera M.
dc.contributor.authorMarques, Isabel
dc.contributor.authorDuro, Nuno
dc.contributor.authorda Costa, Mário
dc.contributor.authorRamalho, José C.
dc.contributor.authorPawlowski, Katharina
dc.contributor.authorManadas, Bruno
dc.contributor.authorPinto Ricardo, Cândido P.
dc.contributor.authorRibeiro-Barros, Ana Isabel
dc.contributor.institutionInstituto de Tecnologia Química e Biológica António Xavier (ITQB)
dc.contributor.institutionGeoBioTec - Geobiociências, Geoengenharias e Geotecnologias
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2021-03-29T22:24:35Z
dc.date.available2021-03-29T22:24:35Z
dc.date.issued2020-01-01
dc.descriptionPOCI-01-0145-FEDER-031999 UID/AGR/04129/2019 UID/GEO/04035/2019 POCI-01-0145-FEDER-402-022125 (ref.: ROTEIRO/0028/2013
dc.description.abstractCasuarina glauca displays high levels of salt tolerance, but very little is known about how this tree adapts to saline conditions. To understand the molecular basis of C. glauca response to salt stress, we have analyzed the proteome from branchlets of plants nodulated by nitrogen-fixing Frankia Thr bacteria (NOD+) and non-nodulated plants supplied with KNO3 (KNO3+), exposed to 0, 200, 400, and 600 mM NaCl. Proteins were identified by Short Gel, Long Gradient Liquid Chromatography coupled to Tandem Mass Spectrometry and quantified by Sequential Window Acquisition of All Theoretical Mass Spectra -Mass Spectrometry. 600 proteins were identified and 357 quantified. Differentially Expressed Proteins (DEPs) were multifunctional and mainly involved in Carbohydrate Metabolism, Cellular Processes, and Environmental Information Processing. The number of DEPs increased gradually with stress severity: (i) from 7 (200 mM NaCl) to 40 (600 mM NaCl) in KNO3+; and (ii) from 6 (200 mM NaCl) to 23 (600 mM NaCl) in NOD+. Protein–protein interaction analysis identified different interacting proteins involved in general metabolic pathways as well as in the biosynthesis of secondary metabolites with different response networks related to salt stress. Salt tolerance in C. glauca is related to a moderate impact on the photosynthetic machinery (one of the first and most important stress targets) as well as to an enhancement of the antioxidant status that maintains cellular homeostasis.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent3767213
dc.identifier.doi10.3390/ijms21010078
dc.identifier.issn1661-6596
dc.identifier.otherPURE: 28003003
dc.identifier.otherPURE UUID: 43751766-3c12-4d8b-9c30-8ba3deee8ca6
dc.identifier.otherScopus: 85076912733
dc.identifier.otherPubMed: 31861944
dc.identifier.otherPubMedCentral: PMC6982049
dc.identifier.otherWOS: 000515378000078
dc.identifier.urihttp://hdl.handle.net/10362/114689
dc.identifier.urlhttps://www.scopus.com/pages/publications/85076912733
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/130960/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/5876/147358/PT
dc.subjectActinorhizal plants
dc.subjectCasuarina glauca
dc.subjectFrankia
dc.subjectProteomics
dc.subjectSalt stress
dc.subjectCatalysis
dc.subjectMolecular Biology
dc.subjectSpectroscopy
dc.subjectComputer Science Applications
dc.subjectPhysical and Theoretical Chemistry
dc.subjectOrganic Chemistry
dc.subjectInorganic Chemistry
dc.titleComparative proteomic analysis of nodulated and non-nodulated casuarina glauca sieb. Ex spreng. grown under salinity conditions using sequential window acquisition of all theoretical mass spectra (SWATH-MS)en
dc.typejournal article
degois.publication.issue1
degois.publication.titleInternational Journal of Molecular Sciences
degois.publication.volume21
dspace.entity.typePublication
oaire.awardNumberPTDC/AGR-FOR/4218/2012
oaire.awardNumberUID/NEU/04539/2013
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-FOR%2F4218%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FNEU%2F04539%2F2013/PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
relation.isProjectOfPublication057d5da9-b16d-4358-8962-590659cecab5
relation.isProjectOfPublication8e21e088-fec1-4e41-9f8e-9a357b2a68bd
relation.isProjectOfPublication.latestForDiscovery057d5da9-b16d-4358-8962-590659cecab5

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