Logo do repositório
 
Publicação

Sustainable development of dual–templating molecularly imprinted polymers for biopurification

2025-03Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorFurtado, Ana I.
dc.contributor.authorLobato, Diogo
dc.contributor.authorBonifácio, Vasco D. B.
dc.contributor.authorViveiros, Raquel
dc.contributor.authorCasimiro, Teresa
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionLAQV@REQUIMTE
dc.contributor.pblElsevier
dc.date.accessioned2025-07-01T21:17:10Z
dc.date.available2025-07-01T21:17:10Z
dc.date.issued2025-03
dc.descriptionFunding Information: The authors would like to thank financial support from Fundação para a Ciência e a Tecnologia, Ministério da Ciência, Tecnologia e Ensino Superior (FCT/MCTES, Portugal), through projects PTDC/EQU–EQU/32473/2017, and PTDC/MEC–ONC/29327/2017. A.I.F. acknowledges her PhD grant (SFRH/BD/150696/2020) in the aim of the International Year of the Periodic Table – a Protocol established between the Portuguese Chemical Society (SPQ) and FCT/MCTES. R.V. would like to acknowledge for her Individual Support from Scientific Employment Stimulus (CEEC–IND, 2020.00377.CEECIND) from FCT/MCTES. The Associate Laboratory Research Unit for Green Chemistry – Clean Technologies and Processes – LAQV–REQUIMTE is financed by national funds from FCT/MCTES (10.54499/LA/P/0008/2020, 10.54499/UIDP/50006/2020, and 10.54499/UIDB/50006/2020). Publisher Copyright: © 2025 The Author(s)
dc.description.abstractThe demand for bio–based products is increasing, but the development of efficient purification processes is lagging. However, typically these processes are expensive, non–specific and/or lack of efficiency. Molecularly imprinted polymers (MIPs) are synthetic affinity materials able to mimic the molecular recognition ability of natural molecules, offering a cost–effective alternative to replace the commercial affinity–driven materials based on proteins and antibodies. In this work, MIPs were developed using supercritical carbon dioxide (scCO2) technology using two approaches: i) one–templating (O–MIPs), using L–leucine (LEU) as template, and ii) a dual–templating (D–MIPs), using LEU and L–lysine (LYS) as templates, to evaluate their potential in the molecular recognition of amino acids in simple and complex aqueous solutions. MIPs produced in scCO2 have already shown good performances in organic and aqueous solutions, for small and non–polar template molecules. Herein, their applicability is extended to amino acids but also proteins. MIPs were produced using 2–vinylpyridine (VP) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as crosslinker under scCO2 conditions. Polymers were obtained as white, ready–to–use dry powders. Their affinity performance was assessed by Static Binding Tests (SBTs) and Solid Phase Extraction (SPE) assays using different amino acids and proteins. The best SBT results was obtained by D–MIP with Qmax = 216 mg LEU + LYS/g D–MIP, and IFmax = 8.3. D–MIP also presented higher binding capacities to adsorb their templated–molecules by a dynamic process (SPE) (Qmax = 79 mg LEU + LYS/g D–MIP), and selectively bind their templates in a solution containing a protein (IFmax = 6.8). The green D–MIP provides a robust, tailor–made and sustainable alternative for biopurification processes.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent10
dc.format.extent4464673
dc.identifier.doi10.1016/j.mtchem.2025.102586
dc.identifier.issn2468-5194
dc.identifier.otherPURE: 115459784
dc.identifier.otherPURE UUID: 29863028-07fa-486a-9589-f126f08799af
dc.identifier.otherScopus: 85217913352
dc.identifier.otherWOS: 001435731800001
dc.identifier.otherORCID: /0000-0002-0449-2343/work/187093212
dc.identifier.otherORCID: /0000-0001-9405-6221/work/207947352
dc.identifier.urihttp://hdl.handle.net/10362/184701
dc.identifier.urlhttps://www.scopus.com/pages/publications/85217913352
dc.identifier.urlhttps://www.webofscience.com/wos/woscc/full-record/WOS:001435731800001
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMEC-ONC%2F29327%2F2017/PT
dc.relationDendrimer-based Report-Eradication Antineoplasic Machines
dc.relationDesenvolvimento sustentável de nanomateriais híbridos para catálise e biopurificação.
dc.relationBe$AFE: Sustainable surface engineering for bacteria-free banknotes
dc.relationinfo:eu-repo/grantAgreement/FCT/Concurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)/LA%2FP%2F0008%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Programático/UIDP%2F50006%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Base/UIDB%2F50006%2F2020/PT
dc.subjectBiorecognition
dc.subjectBioseparation
dc.subjectDual–templating
dc.subjectMolecularly imprinted polymers
dc.subjectSolid–phase extraction
dc.subjectSupercritical carbon dioxide
dc.subjectSynthetic affinity materials
dc.subjectCatalysis
dc.subjectElectronic, Optical and Magnetic Materials
dc.subjectBiomaterials
dc.subjectPolymers and Plastics
dc.subjectColloid and Surface Chemistry
dc.subjectMaterials Chemistry
dc.titleSustainable development of dual–templating molecularly imprinted polymers for biopurificationen
dc.typejournal article
degois.publication.firstPage1
degois.publication.lastPage10
degois.publication.titleMaterials Today Chemistry
degois.publication.volume44
dspace.entity.typePublication
oaire.awardNumberPTDC/MEC-ONC/29327/2017
oaire.awardNumberSFRH/BD/150696/2020
oaire.awardNumber2020.00377.CEECIND/CP1586/CT0033
oaire.awardNumberLA/P/0008/2020
oaire.awardNumberUIDP/50006/2020
oaire.awardNumberUIDB/50006/2020
oaire.awardTitleDendrimer-based Report-Eradication Antineoplasic Machines
oaire.awardTitleDesenvolvimento sustentável de nanomateriais híbridos para catálise e biopurificação.
oaire.awardTitleBe$AFE: Sustainable surface engineering for bacteria-free banknotes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMEC-ONC%2F29327%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F150696%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 3ed/2020.00377.CEECIND%2FCP1586%2FCT0033/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)/LA%2FP%2F0008%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Programático/UIDP%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Base/UIDB%2F50006%2F2020/PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamOE
oaire.fundingStreamCEEC IND 3ed
oaire.fundingStreamConcurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)
oaire.fundingStreamConcurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017/2018) - Financiamento Programático
oaire.fundingStreamConcurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017/2018) - Financiamento Base
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
relation.isProjectOfPublicationfb2ea6ed-57cc-4d69-9a0d-6e453550694f
relation.isProjectOfPublication2cbfd4f9-75cf-484c-9e4b-805bae438951
relation.isProjectOfPublication9d641a96-ada7-41d0-905e-006b29aec0d9
relation.isProjectOfPublication0d547fc3-7e93-4cc6-8682-91e5f6baed55
relation.isProjectOfPublicationae403dd6-3940-45dd-a7c4-04c6bec15552
relation.isProjectOfPublication186df1fb-72e1-4b5e-bb56-1760cf233cbe
relation.isProjectOfPublication.latestForDiscovery186df1fb-72e1-4b5e-bb56-1760cf233cbe

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
Furtado_A._I._et_al._2025_..pdf
Tamanho:
4.26 MB
Formato:
Adobe Portable Document Format
Ver/Abrir

Coleções

Home collection (FCT)