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Robustness of the Process of Nucleoid Exclusion of Protein Aggregates in Escherichia coli

2016-03Artigo científico Acesso aberto
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dc.contributor.authorNeeli-Venkata, Ramakanth
dc.contributor.authorMartikainen, Antti
dc.contributor.authorGupta, Abhishekh
dc.contributor.authorGonçalves, Nadia
dc.contributor.authorFonseca, Jose
dc.contributor.authorRibeiro, André S.
dc.contributor.institutionUNINOVA-Instituto de Desenvolvimento de Novas Tecnologias
dc.contributor.pblAmerican Society for Microbiology
dc.date.accessioned2018-05-09T22:03:53Z
dc.date.available2018-05-09T22:03:53Z
dc.date.issued2016-03
dc.descriptionPortuguese Foundation for Science and Technology provided funding to Jose Fonseca under grant number PTDC/BBB-MET/1084/2012. Academy of Finland vertical bar Biotieteiden ja Ympariston Tutkimuksen Toimikunta (Forskningsradet for Biovetenskap och Miljo) provided funding to Andre S. Ribeiro under grant number 257603.
dc.description.abstractUNLABELLED: Escherichia coli segregates protein aggregates to the poles by nucleoid exclusion. Combined with cell divisions, this generates heterogeneous aggregate distributions in subsequent cell generations. We studied the robustness of this process with differing medium richness and antibiotics stress, which affect nucleoid size, using multimodal, time-lapse microscopy of live cells expressing both a fluorescently tagged chaperone (IbpA), which identifies in vivo the location of aggregates, and HupA-mCherry, a fluorescent variant of a nucleoid-associated protein. We find that the relative sizes of the nucleoid's major and minor axes change widely, in a positively correlated fashion, with medium richness and antibiotic stress. The aggregate's distribution along the major cell axis also changes between conditions and in agreement with the nucleoid exclusion phenomenon. Consequently, the fraction of aggregates at the midcell region prior to cell division differs between conditions, which will affect the degree of asymmetries in the partitioning of aggregates between cells of future generations. Finally, from the location of the peak of anisotropy in the aggregate displacement distribution, the nucleoid relative size, and the spatiotemporal aggregate distribution, we find that the exclusion of detectable aggregates from midcell is most pronounced in cells with mid-sized nucleoids, which are most common under optimal conditions. We conclude that the aggregate management mechanisms of E. coli are significantly robust but are not immune to stresses due to the tangible effect that these have on nucleoid size. IMPORTANCE: Escherichia coli segregates protein aggregates to the poles by nucleoid exclusion. From live single-cell microscopy studies of the robustness of this process to various stresses known to affect nucleoid size, we find that nucleoid size and aggregate preferential locations change concordantly between conditions. Also, the degree of influence of the nucleoid on aggregate positioning differs between conditions, causing aggregate numbers at midcell to differ in cell division events, which will affect the degree of asymmetries in the partitioning of aggregates between cells of future generations. Finally, we find that aggregate segregation to the cell poles is most pronounced in cells with mid-sized nucleoids. We conclude that the energy-free process of the midcell exclusion of aggregates partially loses effectiveness under stressful conditions.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent9
dc.format.extent876322
dc.identifier.doi10.1128/JB.00848-15
dc.identifier.issn0021-9193
dc.identifier.otherPURE: 1264601
dc.identifier.otherPURE UUID: 1ad1af39-c956-46fa-b108-e849c9e24b88
dc.identifier.otherPubMed: 26728194
dc.identifier.otherPubMedCentral: PMC4772596
dc.identifier.otherScopus: 84960429084
dc.identifier.otherWOS: 000371526200005
dc.identifier.otherORCID: /0000-0001-7173-7374/work/45233168
dc.identifier.urihttp://hdl.handle.net/10362/36308
dc.language.isoeng
dc.peerreviewedyes
dc.subjectCell Division
dc.subjectEscherichia coli
dc.subjectEscherichia coli Proteins
dc.subjectGenes, Reporter
dc.subjectLuminescent Proteins
dc.subjectMicroscopy, Fluorescence
dc.subjectProtein Aggregates
dc.subjectStaining and Labeling
dc.subjectTime-Lapse Imaging
dc.subjectJournal Article
dc.subjectResearch Support, Non-U.S. Gov't
dc.titleRobustness of the Process of Nucleoid Exclusion of Protein Aggregates in Escherichia colien
dc.typejournal article
degois.publication.firstPage898
degois.publication.issue6
degois.publication.lastPage906
degois.publication.titleJournal of Bacteriology
degois.publication.volume198
dspace.entity.typePublication
rcaap.rightsopenAccess

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