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Nuclear, mitochondrial, and Wolbachia endosymbiont genomes of Onchocerca lupi, Portugal

2026-02-24Artigo científico Acesso aberto
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dc.contributor.authorLatrofa, Maria Stefania
dc.contributor.authorUrso, Ilenia
dc.contributor.authorNotario, Elisabetta
dc.contributor.authorGissi, Carmela
dc.contributor.authorMaia, Carla
dc.contributor.authorMarzano, Marinella
dc.contributor.authorPesole, Graziano
dc.contributor.authorOtranto, Domenico
dc.contributor.institutionLaboratório Associado de Translacção e Inovação para a Saúde Global - LA Real (Pólo IHMT)
dc.contributor.institutionVector borne diseases and pathogens (VBD)
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.pblAmerican Society for Microbiology
dc.date.accessioned2026-06-22T03:03:01Z
dc.date.available2026-06-22T03:03:01Z
dc.date.issued2026-02-24
dc.descriptionFunding text: This work was supported by funding from the European Union—Next Generation EU, Mission 4, Component 2 Investimento 1.1 “Fondo per il Programma Nazionale di Ricerca e Progetti di Rilevante Interesse Nazionale (PRIN PNRR)" (CUP: B53D23025170001) codice Progetto P2022YFRW9 “New insight on the neglected zoonotic parasite Onchocerca lupi: from -OMICS analyses to diagnosis,” by ELIXIR-IT through the PNRR Project ELIXIRxNextGenIT—ELIXIR x NextGenerationIT: consolidation of the Italian Infrastructure for Omics Data and Bioinformatics (Grant Code IR0000010, CUP: B53C22000690005) and by the “Fondo per la Qualità e l'Internazionalizzazione della Ricerca—D.R. n. 2924 of 29/07/2024” (Bari, Italy). D.O., G.P., and C.G. were partially supported by EU funding within the NextGeneration EU-MUR PNRR Extended Partnership initiative on Emerging Infectious Diseases (project no. PE00000007, INF-ACT). Publisher Copyright: © 2026 Latrofa et al.
dc.description.abstractZoonotic Onchocerca lupi (Spirurida, Onchocercidae) has attracted the interest of the scientific community worldwide, by causing severe ocular infections in domestic animals (dogs, cats) and can infect wild carnivores (wolves, coyotes), as well as humans. Though recent advancements in scientific knowledge have been gained, gaps still remain about the biology of this filarioid, as well as its genetic structure. Based on mitochondrial genes, two highly divergent genotypes were identified, in the Iberian Peninsula (genotype 2) and Europe, Asia, and the United States (genotype 1), meanwhile only a draft nuclear genome of O. lupi from the United States is available. This study aimed to fill knowledge gaps about the genomic characterization of this filarioid and its Wolbachia endosymbiont. This study described the shotgun sequencing of an adult specimen of O. lupi isolated from a dog living in Portugal using the PacBio long-read sequencing technology. Three distinct genomes, such as the nuclear, mitochondrial, and Wolbachia endosymbiont, were assembled and analyzed. The assembled nuclear genome, Olupi_PT2024, exhibited high contiguity, accuracy, and completeness. Pairwise mitogenome comparative analyses among several Onchocerca species corroborated the high divergence between the two genotypes from Portugal and the USA, although the observed differences remained within the range of intra-species variation. The complete genome of the Wolbachia endosymbiont of O. lupi confirmed its classification within supergroup C and its close phylogenetic relationship with Wolbachia endosymbionts associated with the genus Onchocerca. The data on these three genomes may provide valuable resources for understanding the biology, population genetics, and phylogeography of this parasite.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent18
dc.format.extent6675924
dc.identifier.doi10.1128/msphere.00625-25
dc.identifier.issn2379-5042
dc.identifier.otherPURE: 163259404
dc.identifier.otherPURE UUID: b176b814-b539-45f4-9606-5357566d945a
dc.identifier.otherScopus: 105030995316
dc.identifier.otherWOS: 001668811200001
dc.identifier.otherPubMed: 41586614
dc.identifier.otherPubMedCentral: PMC12931274
dc.identifier.otherORCID: /0000-0002-2545-7686/work/218363778
dc.identifier.urihttp://hdl.handle.net/10362/203959
dc.identifier.urlhttps://www.scopus.com/pages/publications/105030995316
dc.language.isoeng
dc.peerreviewedyes
dc.subjectmitochondrial genome
dc.subjectnuclear genome
dc.subjectOnchocerca lupi
dc.subjectPortugal
dc.subjectshotgun sequencing
dc.subjectWolbachia endosymbiont genome
dc.subjectMicrobiology
dc.subjectMolecular Biology
dc.titleNuclear, mitochondrial, and Wolbachia endosymbiont genomes of Onchocerca lupi, Portugalen
dc.typejournal article
degois.publication.firstPage1
degois.publication.issue2
degois.publication.lastPage18
degois.publication.titlemSphere
degois.publication.volume11
dspace.entity.typePublication
rcaap.rightsopenAccess

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