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Underexpression of PPAR gamma is associated with aneuploidy and lower differentiation of thyroid tumours of follicular origin

2009-10Artigo científico Acesso aberto
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dc.contributor.authorEspadinha, C.
dc.contributor.authorPinto, A.E.
dc.contributor.authorLeite, Valeriano
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.pblSpandidos Publications
dc.date.accessioned2022-08-03T22:15:38Z
dc.date.available2022-08-06T00:31:54Z
dc.date.embargoedUntil2022-08-04
dc.date.issued2009-10
dc.descriptionFunding: This study was supported by Fundação para a Ciência e Tecnologia (POCTI/CBO/48922/2002), Portugal.
dc.description.abstractPeroxisome proliferator-activated receptor gamma (PPAR gamma) gene is a nuclear receptor that is involved in thyroid tumourigenesis. Recently, our group has shown that follicular carcinomas underexpressing PPAR gamma protein are more prone to develop distant metastases, to invade locally, to present poorly differentiated areas and to persist after surgery. Aneuploidy is also observed in some thyroid tumours, particularly in the more advanced cases. The aim of the present study was to investigate the association of PPAR gamma expression with the degree of differentiation and ploidy status of benign and malignant thyroid neoplasias. DNA cytometric studies, ploidy and S-phase fraction (SPF) determination, and quantitative RT-PCR analysis of molecular markers specific for thyroid follicular cells, namely Tg (thyroglobulin), TSHR (TSH receptor) and NIS (Na+/I- symporter) were compared between thyroid lesions with positive or negative PPAR gamma protein expression. We observed that PPAR gamma-negative tissues expressed lower levels of Tg mRNA [4.66x10(6) a.u. (arbitrary units) +/- 1.49x10(6)], and were more frequently aneuploid (36%), and presented higher SPF (3.1%+/- 0.4) than PPAR gamma-positive samples (Tg mRNA = 2.54x10(7) a.u. +/- 9.72 x 10(6). P=0.0006; aneuploidy=8%, P=0.0031; SPF=2.2%+/- 0.2, P=0.0430). A similar trend was also observed for TSHR and NIS mRNA, although not reaching statistical significance. This study showed that underexpression of PPAR gamma is associated with poor tumour differentiation, aneuploidy and higher cell proliferative activity. Therapies designed to modulate expression of PPAR gamma may have an impact on the growth of thyroid neoplasias.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent6
dc.format.extent171673
dc.identifier.doi10.3892/or_00000516
dc.identifier.issn1021-335X
dc.identifier.otherPURE: 297971
dc.identifier.otherPURE UUID: 8e88ca9d-6d88-4755-9043-a10103b3ed5a
dc.identifier.otherresearchoutputwizard: 13327
dc.identifier.otherPubMed: 19724872
dc.identifier.otherWOS: 000269766600030
dc.identifier.otherScopus: 70350364844
dc.identifier.urihttp://hdl.handle.net/10362/142826
dc.language.isoeng
dc.peerreviewedyes
dc.subjectNi+/I-symporterACTIVATED RECEPTOR-GAMMA
dc.subjectTSH receptor
dc.subjectFLOW-CYTOMETRY
dc.subjectGROWTH-INHIBITION
dc.subjectthyroglobulin
dc.subjectPPAR gamma
dc.subjectaneuploidy
dc.subjectDNA-BINDING ACTIVITY
dc.subjectthyroid
dc.subjectGENE-EXPRESSION
dc.subjectHUMAN BREAST-CANCER
dc.subjectINDUCE APOPTOSIS
dc.subjectHUMAN COLON-CANCER
dc.subjectTERMINAL DIFFERENTIATION
dc.subjectCARCINOMA-CELLS
dc.titleUnderexpression of PPAR gamma is associated with aneuploidy and lower differentiation of thyroid tumours of follicular originen
dc.typejournal article
degois.publication.firstPage907
degois.publication.issue4
degois.publication.lastPage913
degois.publication.titleOncology Reports
degois.publication.volume22
dspace.entity.typePublication
rcaap.rightsopenAccess

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