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Antimicrobial Resistance Genes in Legionella from Artificial Water Systems

2024-12Artigo científico Acesso aberto
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dc.contributor.authorPereira, Bernardo Beirão
dc.contributor.authorMarrafa, Mário
dc.contributor.authorCruz, Carolina
dc.contributor.authorRodrigues, Lúcia
dc.contributor.authorNunes, Filipa
dc.contributor.authorMonteiro, Silvia
dc.contributor.authorSantos, Ricardo
dc.contributor.authorCarneiro, Rui Neves
dc.contributor.authorNeto, Célia
dc.contributor.authorAguilar, Joana
dc.contributor.authorFerreiro, Nuno Rafael
dc.contributor.authorPassanha, Margarida
dc.contributor.authorCandeias, Gonçalo
dc.contributor.authorFernandes, Aida
dc.contributor.authorPaixão, Paulo
dc.contributor.authorPaixão, Paulo
dc.contributor.authorChasqueira, Maria Jesus
dc.contributor.authorChasqueira, Maria-Jesus
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.institutionComprehensive Health Research Centre (CHRC) - pólo NMS
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2025-01-17T21:22:20Z
dc.date.available2025-01-17T21:22:20Z
dc.date.issued2024-12
dc.description.abstractBackground: Legionella species are the causative agent of Legionnaires’ disease and, as ubiquitous waterborne bacteria, are prone to antimicrobial resistance gene (ARG) acquisition and dissemination due to the antimicrobial contamination of natural environments. Given the potential health risks associated with ARGs, it is crucial to assess their presence in the Legionella population. Methods: The ARGs lpeAB and tet56 were detected in 348 samples, isolates, and DNA extracts using conventional PCR. In a subset of lpeAB-positive isolates, azithromycin (AZT) MIC values were obtained using the EUCAST protocol and LpeAB activity was evaluated through an efflux pump inhibition assay. Results: The lpeAB gene was found in 19% (66/348) of samples, with higher detection rates in the L. pneumophila and L. pneumophila sg1 subgroups, at 30% and 41%, respectively. A positive association between lpeAB and L. pneumophila sg1 was found. The MIC values of the lpeAB-positive isolates ranged from 0.064 to 2 mg/L. LpeAB inhibition resulted in 2- and 4-fold MIC reductions in 10 of the 13 isolates analyzed. One sample each of L. longbeacheae and L. bozemanae was found to possess the tet56 gene. Conclusions: The lpeAB gene is predominant in L. pneumophila sg1. A few isolates with the lpeAB gene exhibited MIC values below the EUCAST tentative highest MIC values for wild-type isolates. Expanding ARG monitoring in Legionella is essential to assess the public health risk of Legionnaires’ disease.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent829191
dc.identifier.doi10.3390/antibiotics13121121
dc.identifier.issn2079-6382
dc.identifier.otherPURE: 105800483
dc.identifier.otherPURE UUID: 1f0662ef-c6af-4fe7-aed2-57cbc2c12c56
dc.identifier.otherScopus: 85213422308
dc.identifier.otherWOS: 001384163700001
dc.identifier.otherPubMed: 39766511
dc.identifier.urihttp://hdl.handle.net/10362/177594
dc.identifier.urlhttps://www.scopus.com/pages/publications/85213422308
dc.language.isoeng
dc.peerreviewedyes
dc.subjectantimicrobial resistance
dc.subjectARG
dc.subjectLegionella
dc.subjectLegionella pneumophila
dc.subjectLpeAB
dc.subjectTet56
dc.subjectMicrobiology
dc.subjectBiochemistry
dc.subjectPharmacology, Toxicology and Pharmaceutics(all)
dc.subjectMicrobiology (medical)
dc.subjectInfectious Diseases
dc.subjectPharmacology (medical)
dc.subjectSDG 3 - Good Health and Well-being
dc.titleAntimicrobial Resistance Genes in Legionella from Artificial Water Systemsen
dc.title.subtitleFindings from a Two-Year Studyen
dc.typejournal article
degois.publication.issue12
degois.publication.titleAntibiotics
degois.publication.volume13
dspace.entity.typePublication
person.familyNamePaixão
person.familyNameChasqueira
person.givenNamePaulo
person.givenNameMaria-Jesus
person.identifier.orcid0000-0002-4294-0872
person.identifier.orcid0000-0002-8015-7109
person.identifier.scopus-author-id6508103792
rcaap.rightsopenAccess
relation.isAuthorOfPublication05b765bd-f025-43ee-8cf5-2e54ceb007cb
relation.isAuthorOfPublication072ec2a7-7dea-429d-bdb3-2d9fa3f1e79d
relation.isAuthorOfPublication.latestForDiscovery072ec2a7-7dea-429d-bdb3-2d9fa3f1e79d

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