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Revisiting in vivo staining with alizarin red S - a valuable approach to analyse zebrafish skeletal mineralization during development and regeneration

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This work was funded in part by European Regional Development Fund (ERDF) through the COMPETE - Operational Competitiveness Program and national funds through FCT - Foundation for Science and Technology, under the project "PEst-C/MAR/LA0015/2011 and UID/Multi/04326/2013. A. Bensimon-Brito acknowledges a FCT PhD fellowship SFRH/40573/2007 and is presently recipient of a fellowship within the iNOVA4Health (UID/Multi/ 04462/2013) project. J. Cardeira is the recipient of a FCT fellowship SFRH/BD/52425/2013, within the ProRegeM PhD Programme, Department of Biomedical Sciences and Medicine from the University of Algarve. G. Dionisio is the recipient of the FCT fellowship SFRH/BD/73205/2010, and A. Huysseune and P.E. Witten acknowledge a grant from FWO 3G.0040.08.

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