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Rhipicephalus bursa Sialotranscriptomic Response to Blood Feeding and Babesia ovis Infection

2018-05-04Artigo científico Acesso aberto
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dc.contributor.authorAntunes, S
dc.contributor.authorCouto, Joana
dc.contributor.authorFerrolho, J
dc.contributor.authorRodrigues, Fábio
dc.contributor.authorNobre, João
dc.contributor.authorSantos, Ana Sofia
dc.contributor.authorSantos-Silva, Maria Margarida
dc.contributor.authorDe La Fuente, José
dc.contributor.authorDomingos, A
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.institutionVector borne diseases and pathogens (VBD)
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.pblFrontiers Media
dc.date.accessioned2021-05-03T22:39:22Z
dc.date.available2021-05-03T22:39:22Z
dc.date.issued2018-05-04
dc.description.abstractTicks are among the most prevalent blood-feeding arthropods, and they act as vectors and reservoirs for numerous pathogens. Sialotranscriptomic characterizations of tick responses to blood feeding and pathogen infections can offer new insights into the molecular interplay occurring at the tick-host-pathogen interface. In the present study, we aimed to identify and characterize Rhipicephalus bursa salivary gland (SG) genes that were differentially expressed in response to blood feeding and Babesia ovis infection. Our experimental approach consisted of RNA sequencing of SG from three different tick samples, fed-infected, fed-uninfected, and unfed-uninfected, for characterization and inter-comparison. Overall, 7,272 expressed sequence tags (ESTs) were constructed from unfed-uninfected, 13,819 ESTs from fed-uninfected, and 15,292 ESTs from fed-infected ticks. Two catalogs of transcripts that were differentially expressed in response to blood feeding and B. ovis infection were produced. Four genes coding for a putative vitellogenin-3, lachesin, a glycine rich protein, and a secreted cement protein were selected for RNA interference functional studies. A reduction of 92, 65, and 51% was observed in vitellogenin-3, secreted cement, and lachesin mRNA levels in SG, respectively. The vitellogenin-3 knockdown led to increased tick mortality, with 77% of ticks dying post-infestation. The reduction of the secreted cement protein-mRNA levels resulted in 46% of ticks being incapable of correctly attaching to the host and significantly lower female weights post-feeding in comparison to the control group. The lachesin knockdown resulted in a 70% reduction of the levels associated with B. ovis infection in R. bursa SG and 70% mortality. These results improved our understanding of the role of tick SG genes in Babesia infection/proliferation and tick feeding. Moreover, lachesin, vitellogenin-3, and secreted cement proteins were validated as candidate protective antigens for the development of novel tick and tick-borne disease control measures.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent17
dc.format.extent2032415
dc.identifier.doi10.3389/fcimb.2018.00116
dc.identifier.issn2235-2988
dc.identifier.otherPURE: 6127751
dc.identifier.otherPURE UUID: 1d23d87a-fd60-4c79-aaf6-3729b37f54b0
dc.identifier.otherScopus: 85046840992
dc.identifier.otherPubMed: 29780749
dc.identifier.otherPubMedCentral: PMC5945973
dc.identifier.otherORCID: /0000-0002-5512-9093/work/70243424
dc.identifier.otherWOS: 000431438000001
dc.identifier.urihttp://hdl.handle.net/10362/116850
dc.language.isoeng
dc.peerreviewedyes
dc.subjectBabesia spp
dc.subjectRNA interference
dc.subjectRhipicephalus bursa
dc.subjectSialotranscriptomics
dc.subjectVaccine
dc.subjectvector-pathogen interactions
dc.subjectMicrobiology
dc.subjectGeneral Immunology and Microbiology
dc.subjectInfectious Diseases
dc.subjectSDG 3 - Good Health and Well-being
dc.titleRhipicephalus bursa Sialotranscriptomic Response to Blood Feeding and Babesia ovis Infectionen
dc.title.subtitleIdentification of Candidate Protective Antigensen
dc.typejournal article
degois.publication.titleFrontiers in Cellular and Infection Microbiology
degois.publication.volume8
dspace.entity.typePublication
rcaap.rightsopenAccess

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