Evidence for selenocysteine coordination to the active site nickel in the [NiFeSe]hydrogenases from Desulfovibrio baculatus.

Eidsness, M. K.; Scott, R. A.; Prickril, B. C.; DerVartanian, D. V.; Legall, J.; Moura, I.; Moura, José J. G.; Peck, H. D.

http://hdl.handle.net/10362/80900

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Ni and Se x-ray absorption spectroscopic studies of the [NiFeSe]hydrogenases from Desulfovibrio baculatus are described. The Ni site geometry is pseudo-octahedral with a coordinating ligand composition of 3-4 (N,O) at 2.06 A, 1-2 (S,Cl) at 2.17 A, and 1 Se at 2.44 A. The Se coordination environment consists of 1 C at 2.0 A and a heavy scatterer M (M = Ni or Fe) at approximately 2.4 A. These results are interpreted in terms of a selenocysteine residue coordinated to the Ni site. The possible role of the Ni-Se site in the catalytic activation of H2 is discussed.

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http://www.scopus.com/inward/record.url?scp=0024485986&partnerID=8YFLogxK
http://hdl.handle.net/10362/80900

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10.1073/pnas.86.1.147

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