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Bioconversion of Terephthalic Acid and Ethylene Glycol Into Bacterial Cellulose by Komagataeibacter xylinus DSM 2004 and DSM 46604

2022-04-05Artigo científico Acesso aberto
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dc.contributor.authorEsmail, Asiyah
dc.contributor.authorRebocho, Ana T.
dc.contributor.authorMarques, Ana C.
dc.contributor.authorSilvestre, Sara
dc.contributor.authorGonçalves, Alexandra
dc.contributor.authorFortunato, Elvira
dc.contributor.authorTorres, Cristiana A. V.
dc.contributor.authorReis, Maria A. M.
dc.contributor.authorFreitas, Filomena
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.institutionUNINOVA-Instituto de Desenvolvimento de Novas Tecnologias
dc.contributor.institutionDCM - Departamento de Ciência dos Materiais
dc.contributor.pblFrontiers Media
dc.date.accessioned2022-08-03T22:26:40Z
dc.date.available2022-08-03T22:26:40Z
dc.date.issued2022-04-05
dc.descriptionLA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy-i4HB, the project LA/P/0037/2020 of the Associate Laboratory Institute of Nanostructures, Nanomodelling and Nanofabrication i3N, and by the European Union’s Horizon 2020 research and innovation program through Project Bio Innovation of a Circular Economy for Plastics (BioICEP), under grant agreement No. 870292, supported by the National Natural Science Foundation of China (grant numbers: Institute of Microbiology, Chinese Academy of Sciences: 31961133016; Beijing Institute of Technology: 31961133015; Shandong University: 31961133014). AE and AR acknowledge FCT I.P. for PhD Grants 2021.05014. BD and 2020.06470. BD, respectively. Publisher Copyright: Copyright © 2022 Esmail, Rebocho, Marques, Silvestre, Gonçalves, Fortunato, Torres, Reis and Freitas.
dc.description.abstractKomagataeibacter xylinus strains DSM 2004 and DSM 46604 were evaluated for their ability to grow and produce bacterial cellulose (BC) upon cultivation on terephthalic acid (TA) and ethylene glycol (EG), which are monomers of the petrochemical-derived plastic polyethylene terephthalate (PET). Both strains were able to utilize TA, EG, and their mixtures for BC synthesis, with different performances. K. xylinus DSM 2004 achieved higher BC production from TA (0.81 ± 0.01 g/L), EG (0.64 ± 0.02 g/L), and TA + EG mixtures (0.6 ± 0.1 g/L) than strain DSM 46604. The latter was unable to utilize EG as the sole carbon source and reached a BC production of 0.16 ± 0.01 g/L and 0.23 ± 0.1 g/L from TA alone or TA + EG mixtures, respectively. Further supplementing the media with glucose enhanced BC production by both strains. During cultivation on media containing TA and EG, rapid pH drop due to metabolization of EG into acidic compounds led to some precipitation of TA that was impregnated into the BC pellicles. An adaptation of the downstream procedure involving BC dissolution in NaOH was used for the recovery of pure BC. The different medium composition tested, as well as the downstream procedure, impacted the BC pellicles’ physical properties. Although no variation in terms of the chemical structure were observed, differences in crystallinity degree and microstructure of the produced BC were observed. The BC produced by K. xylinus DSM 2004 had a higher crystallinity (19–64%) than that of the strain DSM 46604 (17–53%). Moreover, the scanning electron microscopy analysis showed a higher fiber diameter for K. xylinus DSM 2004 BC (46–56 nm) than for K. xylinus DSM 46604 (37–49 nm). Dissolution of BC in NaOH did not influence the chemical structure; however, it led to BC conversion from type I to type II, as well as a decrease in crystallinity. These results demonstrate that PET monomers, TA and EG, can be upcycled into a value-added product, BC, presenting an approach that will contribute to lessening the environmental burden caused by plastic disposal in the environment.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent11
dc.format.extent2424066
dc.identifier.doi10.3389/fbioe.2022.853322
dc.identifier.issn2296-4185
dc.identifier.otherPURE: 44762073
dc.identifier.otherPURE UUID: e8d1dac7-3366-4041-87cc-72b1db63f374
dc.identifier.otherScopus: 85128716581
dc.identifier.otherPubMed: 35480983
dc.identifier.otherPubMedCentral: PMC9036990
dc.identifier.otherWOS: 000792694800001
dc.identifier.otherORCID: /0000-0002-9430-4640/work/116781862
dc.identifier.otherORCID: /0000-0002-4202-7047/work/116781970
dc.identifier.urihttp://hdl.handle.net/10362/142843
dc.identifier.urlhttps://www.scopus.com/pages/publications/85128716581
dc.language.isoeng
dc.peerreviewedyes
dc.relationFunding Information: info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
dc.relationApplied Molecular Biosciences Unit
dc.subjectbacterial cellulose
dc.subjectbioconversion
dc.subjectethylene glycol
dc.subjectPET
dc.subjectterephthalic acid
dc.subjectBiotechnology
dc.subjectBioengineering
dc.subjectHistology
dc.subjectBiomedical Engineering
dc.titleBioconversion of Terephthalic Acid and Ethylene Glycol Into Bacterial Cellulose by Komagataeibacter xylinus DSM 2004 and DSM 46604en
dc.typejournal article
degois.publication.firstPage1
degois.publication.lastPage11
degois.publication.titleFrontiers in Bioengineering and Biotechnology
degois.publication.volume10
dspace.entity.typePublication
oaire.awardNumberUIDB/04378/2020
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
relation.isProjectOfPublicatione07cf232-4705-4b5b-b2c4-af8f25311076
relation.isProjectOfPublication.latestForDiscoverye07cf232-4705-4b5b-b2c4-af8f25311076

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