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A simplified protocol for Agrobacterium-mediated transformation of cell suspension cultures of the model species Medicago truncatula A17

2023-06Artigo científico Acesso aberto
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dc.contributor.authorFerreira, Ana Clara
dc.contributor.authorRebelo, Bárbara A.
dc.contributor.authorAbranches, Rita
dc.contributor.institutionInstituto de Tecnologia Química e Biológica António Xavier (ITQB)
dc.contributor.pblSpringer Science Business Media
dc.date.accessioned2023-09-29T22:22:09Z
dc.date.available2023-09-29T22:22:09Z
dc.date.issued2023-06
dc.descriptionFunding Information: Open access funding provided by FCT|FCCN (b-on). This work was supported by Fundação para a Ciência e Tecnologia (FCT, Portugal) through Research Units “Molecular, Structural and Cellular Microbiology” (MOSTMICRO, UIDB/ 04612/2020) and “Bioresources 4 Sustainability” (GREEN-IT, UIDB/04551/2020). Publisher Copyright: © 2023, The Author(s).
dc.description.abstractThis manuscript describes a unique protocol for the rapid transformation of Medicago truncatula A17 cell suspension cultures mediated by Agrobacterium tumefaciens. Medicago cells were collected on day 7 of the growth curve, which corresponded to the beginning of the exponential phase. They were then co-cultured with Agrobacterium for 3 days before being spread onto a petri dish with appropriate antibiotic selection. The Receptor Binding Domain of the Spike protein of SARS-CoV-2 was used as a model to develop this protocol. The presence of the transgene was assessed using PCR, and the integrity of the product was evaluated by SDS-PAGE and Western-blotting.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent7
dc.format.extent965986
dc.identifier.doi10.1007/s11240-023-02495-6
dc.identifier.issn0167-6857
dc.identifier.otherPURE: 72725949
dc.identifier.otherPURE UUID: eabf0290-7682-47bc-8fa6-5ed12b4a43e4
dc.identifier.otherScopus: 85150766118
dc.identifier.urihttp://hdl.handle.net/10362/158481
dc.identifier.urlhttps://www.scopus.com/pages/publications/85150766118
dc.language.isoeng
dc.peerreviewedyes
dc.subjectAgrobacterium
dc.subjectCultured cells
dc.subjectMedicago
dc.subjectModel legume
dc.subjectPlant-based system
dc.subjectRBD
dc.subjectSARS-CoV-2
dc.subjectStable transformation
dc.subjectHorticulture
dc.titleA simplified protocol for Agrobacterium-mediated transformation of cell suspension cultures of the model species Medicago truncatula A17en
dc.typejournal article
degois.publication.firstPage669
degois.publication.issue3
degois.publication.lastPage675
degois.publication.titlePlant Cell, Tissue and Organ Culture
degois.publication.volume153
dspace.entity.typePublication
rcaap.rightsopenAccess

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