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Natural Deep Eutectic Solvent (NADES) Extraction Improves Polyphenol Yield and Antioxidant Activity of Wild Thyme (Thymus serpyllum L.) Extracts

2022-02Artigo científico Acesso aberto
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dc.contributor.authorPavlić, Branimir
dc.contributor.authorMrkonjić, Živan
dc.contributor.authorTeslić, Nemanja
dc.contributor.authorKljakić, Aleksandra Cvetanović
dc.contributor.authorPojić, Milica
dc.contributor.authorMandić, Anamarija
dc.contributor.authorStupar, Alena
dc.contributor.authorSantos, Filipa
dc.contributor.authorDuarte, Ana Rita C.
dc.contributor.authorMišan, Aleksandra
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionLAQV@REQUIMTE
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2022-09-16T22:28:56Z
dc.date.available2022-09-16T22:28:56Z
dc.date.issued2022-02
dc.descriptionFunding Information: info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT Funding: This research was supported by the Science Fund of the Republic of Serbia, 6060592, Natural Deep Eutectic Solvents for Green Agri-Food Solutions—DEStiny and 7750168, Novel extracts and bioactive compounds from under-utilized resources for high-value applications—BioUtilize. This project received funding from the European Union’s Horizon 2020—European Research Council (ERC)—under grant agreement No ERC-2016-CoG 725034. T Funding Information: This research was supported by the Science Fund of the Republic of Serbia, 6060592, Natural Deep Eutectic Solvents for Green Agri-Food Solutions?DEStiny and 7750168, Novel extracts and bioactive compounds from under-utilized resources for high-value applications?BioUtilize. This project received funding from the European Union?s Horizon 2020?European Research Council (ERC)?under grant agreement No ERC-2016-CoG 725034. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
dc.description.abstractWild thyme (Thymus serpyllum L.) herbal dust has been recognized as a potential underutilized resource for the recovery of antioxidants. The aim of this paper was to optimize natural deep eutectic solvent (NADES) extraction of polyphenols to obtain improved antioxidant activity of extracts determined by selected in vitro assays (DPPH, FRAP, and ABTS). Twenty different NADES systems were investigated in the first step of the screening of the extraction solvent and L-proline (Pro)–glycerine (Gly) based solvents provided the best results. Preliminary experiments organized by 25−1 fractional factorial design narrowed down the number of extraction factors from five (temperature, extraction time, NADES type, water content and L/S ratio) to three and determined their experimental domain for the final step. A face-centered central composite design with temperature (40–55–70 °C), extraction time (60–120–180 min) and L/S ratio (10–20–30 g NADES/g sample) was applied for influence analysis and process optimization. Multi-response optimization suggested a temperature of 65 °C, time of extraction of 180 min and L/S ratio of 28 g NADES/g DW as optimal extraction parameters. Experimental validation confirmed good agreement between experimental and predicted results in the extract obtained at optimal conditions and the interactions in the most suitable NADES (N16; Pro–Gly–H2O; 1:2:1) were confirmed by the1H-NMR.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent20
dc.format.extent1253403
dc.identifier.doi10.3390/molecules27051508
dc.identifier.issn1420-3049
dc.identifier.otherPURE: 46583301
dc.identifier.otherPURE UUID: 28a7db5e-d9f1-402d-88c8-b2566d2b771e
dc.identifier.otherScopus: 85125173144
dc.identifier.otherPubMed: 35268607
dc.identifier.otherPubMedCentral: PMC8911718
dc.identifier.otherWOS: 000775091300001
dc.identifier.otherORCID: /0000-0003-0800-0112/work/119162268
dc.identifier.urihttp://hdl.handle.net/10362/143805
dc.identifier.urlhttps://www.scopus.com/pages/publications/85125173144
dc.language.isoeng
dc.peerreviewedyes
dc.subjectAntioxidant activity
dc.subjectNADES
dc.subjectPolyphenols
dc.subjectRSM optimization
dc.subjectThymus serpyllum L
dc.subjectAnalytical Chemistry
dc.subjectChemistry (miscellaneous)
dc.subjectMolecular Medicine
dc.subjectPharmaceutical Science
dc.subjectDrug Discovery
dc.subjectPhysical and Theoretical Chemistry
dc.subjectOrganic Chemistry
dc.titleNatural Deep Eutectic Solvent (NADES) Extraction Improves Polyphenol Yield and Antioxidant Activity of Wild Thyme (Thymus serpyllum L.) Extractsen
dc.typejournal article
degois.publication.issue5
degois.publication.titleMolecules
degois.publication.volume27
dspace.entity.typePublication
rcaap.rightsopenAccess

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