Logo do repositório
 
Publicação

Enhanced primers for amplification of DNA barcodes from a broad range of marine metazoans

2013-09-10Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorLobo, Jorge
dc.contributor.authorCosta, Pedro M.
dc.contributor.authorTeixeira, Marcos A. L.
dc.contributor.authorFerreira, Maria S. G.
dc.contributor.authorCosta, Maria Helena Ferrão Ribeiro da
dc.contributor.authorCosta, Filipe Oliveira
dc.contributor.institutionDCEA - Departamento de Ciências e Engenharia do Ambiente
dc.contributor.pblSpringer Verlag
dc.date.accessioned2018-03-02T23:00:30Z
dc.date.available2018-03-02T23:00:30Z
dc.date.issued2013-09-10
dc.descriptionThis work was supported by FEDER through POFC-COMPETE and by national funds from "Fundacao para a Ciencia e a Tecnologia (FCT)" in the scope of the grants FCOMP-01-0124-FEDER-015429. Filipe O Costa benefitted from the Marie Curie European Reintegration Grant PERG02-GA-2007-224890, provided by the European Commission. The authors would also like to thank Carolina Madeira and Monica Landi for the initial molecular experiments, Luisa Borges and Ronaldo Sousa for the support on collections, Catia Bartilotti (IPMA), Nuno Gomes (CBMA) for the offer of crustacean specimens and Artur Oliveira (CBMA) for the alga specimen.
dc.description.abstractBACKGROUND: Building reference libraries of DNA barcodes is relatively straightforward when specifically designed primers are available to amplify the COI-5P region from a relatively narrow taxonomic group (e.g. single class or single order). DNA barcoding marine communities have been comparatively harder to accomplish due to the broad taxonomic diversity and lack of consistently efficient primers. Although some of the so-called "universal" primers have been relatively successful, they still fail to amplify COI-5P of many marine animal groups, while displaying random success even among species within each group. Here we propose a new pair of primers designed to enhance amplification of the COI-5P region in a wide range of marine organisms. RESULTS: Amplification tests conducted on a wide range of marine animal taxa, rendered possible the first--time sequencing of DNA barcodes from eight separated phyla (Annelida, Arthropoda, Chordata, Cnidaria, Echinodermata, Mollusca, Nemertea and Platyhelminthes), comprising a total of 14 classes, 28 orders, 57 families, 68 genus and 76 species. CONCLUSIONS: These primers demonstrated to be highly cost-effective, which is of key importance for DNA barcoding procedures, such as for building comprehensive DNA barcode libraries of marine communities, where the processing of a large numbers of specimens from a wide variety of marine taxa is compulsory.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent782793
dc.identifier.doi10.1186/1472-6785-13-34
dc.identifier.issn1472-6785
dc.identifier.otherPURE: 258950
dc.identifier.otherPURE UUID: c4fc550a-9ee8-42e1-8a2e-fabc93ca74f7
dc.identifier.otherresearchoutputwizard: 39323
dc.identifier.otherPubMed: 24020880
dc.identifier.otherScopus: 84883611456
dc.identifier.otherWOS: 000324237100001
dc.identifier.otherPubMedCentral: PMC3846737
dc.identifier.otherORCID: /0000-0001-8479-4941/work/50174068
dc.identifier.urihttp://hdl.handle.net/10362/31568
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FBIA%2FUI4050%2F2011/PT
dc.relationStrategic Project - UI 4050 - 2011-2012
dc.relationMOLECULAR BIODIVERSITY AND ADVANCED MONITORING TOOLS FOR MACROBENTHIC COMMUNITIES OF PORTUGUESE COASTAL ECOSYSTEMS
dc.relationinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F72564%2F2010/PT
dc.subjectDNA barcoding
dc.subjectMarine metazoans
dc.subjectTaxonomy
dc.subjectUniversal primers
dc.subjectSDG 14 - Life Below Water
dc.titleEnhanced primers for amplification of DNA barcodes from a broad range of marine metazoansen
dc.typejournal article
degois.publication.titleBmc Ecology
degois.publication.volume13
dspace.entity.typePublication
oaire.awardNumberPEst-C/BIA/UI4050/2011
oaire.awardNumberSFRH/BD/69750/2010
oaire.awardNumberSFRH/BPD/72564/2010
oaire.awardTitleStrategic Project - UI 4050 - 2011-2012
oaire.awardTitleMOLECULAR BIODIVERSITY AND ADVANCED MONITORING TOOLS FOR MACROBENTHIC COMMUNITIES OF PORTUGUESE COASTAL ECOSYSTEMS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FBIA%2FUI4050%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F69750%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F72564%2F2010/PT
oaire.fundingStream6820 - DCRRNI ID
oaire.fundingStreamFARH
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
relation.isProjectOfPublicatione35c24d5-1360-4da3-991b-e5e296d4bd01
relation.isProjectOfPublication765b16d8-20d7-450d-8dea-b2e0477e47a9
relation.isProjectOfPublication77ee1969-51a3-4568-8542-ee1837ed53c4
relation.isProjectOfPublication.latestForDiscoverye35c24d5-1360-4da3-991b-e5e296d4bd01

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
1472-6785-13-34.pdf
Tamanho:
764.45 KB
Formato:
Adobe Portable Document Format
Ver/Abrir

Coleções

FCT: DCSA - Artigos em revista internacional com arbitragem científica