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Clonal lineages, antimicrobial resistance, and pvl carriage of staphylococcus aureus associated to skin and soft-tissue infections from ambulatory patients in Portugal

2021-04Artigo científico Acesso aberto
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dc.contributor.authorFerreira, Carolina
dc.contributor.authorCosta, Sofia Santos
dc.contributor.authorSerrano, Maria
dc.contributor.authorOliveira, Ketlyn
dc.contributor.authorTrigueiro, Graça
dc.contributor.authorPomba, Constança
dc.contributor.authorCouto, Isabel
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.institutionTB, HIV and opportunistic diseases and pathogens (THOP)
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2021-05-03T22:56:04Z
dc.date.available2021-05-03T22:56:04Z
dc.date.issued2021-04
dc.description.abstractStaphylococcus aureus (S. aureus) is a leading cause of skin and soft-tissue infections (SSTIs) in the community. In this study, we characterized a collection of 34 S. aureus from SSTIs in ambulatory patients in Portugal and analyzed the presence of Panton–Valentine leucocidin (PVL)-encoding genes and antibiotic-resistance profile, which was correlated with genetic determinants, plasmid carriage, and clonal lineage. Nearly half of the isolates (15, 44.1%) were methicillin-resistant Staphylococcus aureus (MRSA) and/or multidrug resistant (MDR). We also detected resistance to penicillin (33/34, 97.1%), fluoroquinolones (17/34, 50.0%), macrolides and lincosamides (15/34, 44.1%), aminoglycosides (6/34, 17.6%), and fusidic acid (2/34, 5.9%), associated with several combinations of resistance determinants (blaZ, erm(A), erm(C), msr(A), mph(C), aacA-aphD, aadD, aph(3′ )-IIIa, fusC), or mutations in target genes (fusA, grlA/gyrA). The collection presented a high genetic diversity (Simpson’s index of 0.92) with prevalence of clonal lineages CC5, CC22, and CC8, which included the MRSA and also most MDR isolates (CC5 and CC22). PVL-encoding genes were found in seven isolates (20.6%), three methicillin-susceptible Staphylococcus aureus (MSSA) (ST152-agrI and ST30-agrIII), and four MRSA (ST8-agrI). Plasmid profiling revealed seventeen distinct plasmid profiles. This work highlights the high frequency of antimicrobial resistance and PVL carriage in SSTIs-related S. aureus outside of the hospital environment.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent1309934
dc.identifier.doi10.3390/antibiotics10040345
dc.identifier.issn2079-6382
dc.identifier.otherPURE: 29523931
dc.identifier.otherPURE UUID: 089370dc-8ac7-40d6-bcea-36d5e8bbffad
dc.identifier.otherScopus: 85103846152
dc.identifier.otherORCID: /0000-0003-3536-2733/work/93243082
dc.identifier.otherORCID: /0000-0002-4096-2410/work/93243472
dc.identifier.urihttp://hdl.handle.net/10362/116909
dc.identifier.urlhttps://www.scopus.com/pages/publications/85103846152
dc.language.isoeng
dc.peerreviewedyes
dc.subjectAntibiotic resistance
dc.subjectClonal lineages
dc.subjectPanton–Valentine leucocidin
dc.subjectPlasmids
dc.subjectSkin and soft-tissue infections
dc.subjectStaphylococcus aureus
dc.subjectMicrobiology
dc.subjectPharmacology, Toxicology and Pharmaceutics(all)
dc.subjectMicrobiology (medical)
dc.subjectInfectious Diseases
dc.subjectPharmacology (medical)
dc.subjectSDG 3 - Good Health and Well-being
dc.subjectSDG 12 - Responsible Consumption and Production
dc.titleClonal lineages, antimicrobial resistance, and pvl carriage of staphylococcus aureus associated to skin and soft-tissue infections from ambulatory patients in Portugalen
dc.typejournal article
degois.publication.issue4
degois.publication.titleAntibiotics
degois.publication.volume10
dspace.entity.typePublication
rcaap.rightsopenAccess

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