Logo do repositório
 
Publicação

Harmonization of IgG antibody values against the SARS-CoV-2 Spike protein

2026-12Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorSaraiva, Ana Leonor
dc.contributor.authorAfreixo, Vera
dc.contributor.authorAmaral, Palmira
dc.contributor.authorViana, João Faro
dc.contributor.authorAntunes, Isabel
dc.contributor.authorPaiva, Artur
dc.contributor.authorGonçalves, Lígia Antunes
dc.contributor.authorAraújo, Lucília
dc.contributor.authorMachado, Ausenda
dc.contributor.authorGaio, Vânia
dc.contributor.institutionLaboratório Associado de Translacção e Inovação para a Saúde Global - LA Real (Pólo ENSP)
dc.contributor.institutionComprehensive Health Research Centre (CHRC) - Pólo ENSP
dc.contributor.institutionCentro de Investigação em Saúde Pública (CISP/PHRC)
dc.contributor.institutionEscola Nacional de Saúde Pública (ENSP)
dc.contributor.pblNature Publishing Group
dc.date.accessioned2026-05-29T10:22:01Z
dc.date.available2026-05-29T10:22:01Z
dc.date.issued2026-12
dc.descriptionPublisher Copyright: © The Author(s) 2026.
dc.description.abstractThe COVID-19 pandemic led to the rapid development of vaccines and diagnostic tests. To evaluate the response of IgG antibodies against the SARS-CoV-2 Spike protein in healthcare workers, a cohort study (2020–2022) was conducted in three Portuguese hospitals. Each hospital used a different assay (Abbott CMIA, Roche Elecsys ECLIA, Siemens ADVIA Centaur), which presents challenges to comparability. This study aimed to harmonize anti-S IgG levels across hospitals using a mathematical approach for combined analysis, and to estimate anti-S IgG levels over time, thus better understanding immunity dynamics. Antibody levels were measured at six time points: before vaccination, after full vaccination, 3, 6, and 12 months after the second dose, and after booster. Various harmonization strategies were tested, including WHO international conversion and quantile interpolation, followed by Deming regression. Quantile interpolation plus regression proved to be more effective than WHO conversion, preserving each hospital’s data distribution and aligning the harmonized values with the Abbott CMIA used as the reference scale. After harmonization, antibody titers followed the expected trend: a sharp rise post-vaccination, a gradual decline over 12 months (659.32 BAU/mL before booster), and a notable increase after the booster (25,919.89 BAU/mL). Although cross-laboratory validation was lacking, this approach proved practical, reproducible, and promising for multicenter studies that integrate serological data from diverse platforms, at the European and global levels.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent30401309
dc.identifier.doi10.1038/s41598-026-37341-0
dc.identifier.issn2045-2322
dc.identifier.otherPURE: 163881474
dc.identifier.otherPURE UUID: 1a6597d1-1298-4288-b2ef-71eeacd10dad
dc.identifier.otherScopus: 105033827586
dc.identifier.otherPubMed: 41708677
dc.identifier.otherPubMedCentral: PMC13004943
dc.identifier.otherWOS: 001719788700012
dc.identifier.urihttp://hdl.handle.net/10362/203575
dc.identifier.urlhttps://www.scopus.com/pages/publications/105033827586
dc.language.isoeng
dc.peerreviewedyes
dc.subjectDeming regression
dc.subjectHarmonization
dc.subjectHealthcare workers
dc.subjectIgG antibody
dc.subjectQuantile interpolation
dc.subjectSARS-CoV-2
dc.subjectGeneral
dc.subjectSDG 3 - Good Health and Well-being
dc.titleHarmonization of IgG antibody values against the SARS-CoV-2 Spike proteinen
dc.typejournal article
degois.publication.issue1
degois.publication.titleScientific Reports
degois.publication.volume16
dspace.entity.typePublication
rcaap.rightsopenAccess

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
Saraiva_2026_Scientific_Reports_16_9522.pdf
Tamanho:
28.99 MB
Formato:
Adobe Portable Document Format
Ver/Abrir

Coleções

Home collection (ENSP)