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Collagen–Chitosan Composites Enhanced with Hydroxytyrosol for Prospective Wound Healing Uses

2025-05-06Artigo científico Acesso aberto
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dc.contributor.authorBatista, Miguel P.
dc.contributor.authorPimenta, Margarida
dc.contributor.authorFernández, Naiara
dc.contributor.authorDuarte, Ana Rita C.
dc.contributor.authorBronze, Maria do Rosário
dc.contributor.authorMarto, Joana
dc.contributor.authorGaspar, Frédéric Bustos
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionLAQV@REQUIMTE
dc.contributor.institutionInstituto de Tecnologia Química e Biológica António Xavier (ITQB)
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2025-07-30T21:20:33Z
dc.date.available2025-07-30T21:20:33Z
dc.date.issued2025-05-06
dc.descriptionFunding Information: This research was funded by the Research Unit UID/04462: iNOVA4Health—Programme in Translational Medicine, the iMed.ULisboa–UID 04138, J. Marto–CEECINST/00145/2018 programs, which are financially supported by the Fundação para a Ciência e Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior through national funds. Financial support received from The Associate Laboratory LS4FUTURE was supported by the Fundação para a Ciência e a Tecnologia (FCT, Portugal) through the funding LA/P/0087/2020 (DOI 10.54499/LA/P/0087/2020). This work also received funding from the ERC-2016-CoG 725034 and was supported by the Associate Laboratory for Green Chemistry (LAQV), financed by national funds from FCT/MCTES (LA/P/0008/2020 DOI 10.54499/LA/P/0008/2020, UIDP/50006/2020 DOI 10.54499/UIDP/50006/2020 and UIDB/50006/2020 DOI 10.54499/UIDB/50006/2020). The authors acknowledge the financial support from the Research Unit UID/04462: iNOVA4Health—Programme in Translational Medicine, the iMed.ULisboa–UID 04138, J. Marto–CEECINST/00145/2018 programs and The Associate Laboratory LS4FUTURE supported by the Fundação para a Ciência e a Tecnologia. This work was also supported by the Associate Laboratory for Green Chemistry (LAQV). The co-author Miguel P. Batista acknowledges FCT for financial support through 2020.05895.BD grant. Publisher Copyright: © 2025 by the authors.
dc.description.abstractBackground/Objectives: Recent studies highlight the excellent wound-healing properties of collagen and chitosan materials. Combining these polymers with a bioactive compound could enhance their effectiveness as next-generation wound dressings. Hydroxytyrosol (HT), an antioxidant derived from olive oil, may aid wound healing due to its anti-inflammatory, antimicrobial, and angiogenesis-stimulating properties, making it a beneficial addition to collagen–chitosan dressings. It could be a beneficial addition to collagen–chitosan dressings, thus improving their therapeutic effects. This study screens the potential of collagen–chitosan composites with HT for wound-healing applications and assesses the influence of the compound’s incorporation on the materials’ properties. Methods: The material production involved incorporating chitosan and HT into a marine collagen extract. The resulting collagen–chitosan–HT material was obtained through freeze-drying. Prototype dressing characterization included morphology by scanning electron microscopy, solid and hydrated state by textural and rheological studies, and in vitro HT release studies. The materials’ cytocompatibility screening was assessed using a mouse fibroblast cell line, and the antibacterial activity was evaluated against microorganisms commonly implicated in wound infections. Results: Burst strength, viscosity, frequency sweep test, tackiness, and adhesion results indicate that chitosan contributes to the material’s mechanical robustness by maintaining a high viscosity and preserving the material’s gel structure. The in vitro release studies suggest an HT-controlled release profile with a maximum release (70%) achieved after 10 h. Biological experiments confirmed the materials’ cytocompatibility with skin cells and very promising antibacterial efficacy against Staphylococcus aureus and Pseudomonas aeruginosa. Conclusions: In conclusion, HT was successfully incorporated into a collagen–chitosan matrix, enhancing the therapeutic prospect of the resultant material. The collagen–chitosan–HT composite presents a promising potential as an advanced wound-healing material.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent18
dc.format.extent14393160
dc.identifier.doi10.3390/pharmaceutics17050618
dc.identifier.issn1999-4923
dc.identifier.otherPURE: 123408024
dc.identifier.otherPURE UUID: 4c88bc3d-f344-42c1-9422-9c72d4adbc8e
dc.identifier.otherScopus: 105006824386
dc.identifier.otherWOS: 001495700800001
dc.identifier.otherPubMed: 40430909
dc.identifier.otherPubMedCentral: PMC12114697
dc.identifier.otherORCID: /0000-0003-0800-0112/work/188937140
dc.identifier.urihttp://hdl.handle.net/10362/185773
dc.identifier.urlhttps://www.scopus.com/pages/publications/105006824386
dc.identifier.urlhttps://www.webofscience.com/wos/woscc/full-record/WOS:001495700800001
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/Concurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)/LA%2FP%2F0087%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Programático/UIDP%2F50006%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Base/UIDB%2F50006%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT//2020.05895.BD/PT
dc.relationMarine waste streams valorisation for the production of novel bioactive 3D structures and their evaluation for potential biomedical and food industry applications
dc.subjectAdvanced wound dressings
dc.subjectBiomaterials characterization
dc.subjectBiomedical applications
dc.subjectChitosan
dc.subjectCollagen
dc.subjectHydroxytyrosol
dc.subjectPharmaceutical Science
dc.subjectSDG 14 - Life Below Water
dc.titleCollagen–Chitosan Composites Enhanced with Hydroxytyrosol for Prospective Wound Healing Usesen
dc.typejournal article
degois.publication.firstPage1
degois.publication.issue5
degois.publication.lastPage18
degois.publication.titlePharmaceutics
degois.publication.volume17
dspace.entity.typePublication
oaire.awardNumberLA/P/0087/2020
oaire.awardNumberUIDP/50006/2020
oaire.awardNumberUIDB/50006/2020
oaire.awardNumber2020.05895.BD
oaire.awardTitleMarine waste streams valorisation for the production of novel bioactive 3D structures and their evaluation for potential biomedical and food industry applications
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)/LA%2FP%2F0087%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Programático/UIDP%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Base/UIDB%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2020.05895.BD/PT
oaire.fundingStreamConcurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)
oaire.fundingStreamConcurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017/2018) - Financiamento Programático
oaire.fundingStreamConcurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017/2018) - Financiamento Base
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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project.funder.nameFundação para a Ciência e a Tecnologia
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