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Predicting sample heating induced by cantilevers illuminated by intense light beams

2022-08Artigo científico Acesso aberto
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dc.contributor.authorTremoço, Frederico
dc.contributor.authorGómez-Varela, Ana I.
dc.contributor.authorMiranda, Adelaide
dc.contributor.authorLopez-Garcia, Martin
dc.contributor.authorSilva, Ana G.
dc.contributor.authorDe Beule, Pieter A.A.
dc.contributor.institutionDF – Departamento de Física
dc.contributor.institutionCeFITec – Centro de Física e Investigação Tecnológica
dc.contributor.pblElsevier BV
dc.date.accessioned2022-11-28T22:12:30Z
dc.date.available2022-11-28T22:12:30Z
dc.date.issued2022-08
dc.descriptionAna I. Gómez Varela acknowledges support from the Xunta de Galicia, Consellería de Cultura, Educación e Ordenación Universitaria e da Consellería de Economía, and Emprego e Industria (postdoctoral contracts ED481D-2021-019 and ED481B 2017/103). Adelaide Miranda and Pieter A. A. De Beule acknowledge financial support from Norte's Regional Operational Programme 2014-2020-Norte2020 (NORTE-1-145-FEDER-19). Publisher Copyright: © 2022
dc.description.abstractHybrid microscopy based on Atomic Force Microscopy (AFM) and fluorescence microscopy represents a commonplace experimental approach to study cell biology processes in liquid media at physiological temperature. However, many types of experimental artifacts can arise depending on the fluorescence illumination and detection technique utilized. For example, fluorescence excitation light gets absorbed by AFM cantilevers inducing local heating provoking undesirable as well as uncontrollable cantilever deflections. Here we present a numerical modelling approach based on a Finite Element Model (FEM) to predict sample heating in liquid media quantitatively, depending on illumination wavelength, illumination pattern, and cantilever shape and composition. Modelling results indicate substantial local temperature increases in-line with temperature increases derived from experimental cantilever deflections induced by fluorescence excitation light. We predict temperature increases of ∼0.05 – 0.5 °C for wide-field illumination and ∼5 – 15 °C for confocal illumination within the boundary conditions established, which could, for example, induce local protein conformational changes. We conclude that sample heating is an important issue requiring consideration in experimental set-ups involving intense light illumination of AFM cantilevers, especially when conducting single molecule investigations.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent6
dc.format.extent3090789
dc.identifier.doi10.1016/j.rinp.2022.105718
dc.identifier.issn2211-3797
dc.identifier.otherPURE: 45392216
dc.identifier.otherPURE UUID: 0fc9f90b-85b5-4770-ae9c-7f62e0103570
dc.identifier.otherScopus: 85131968606
dc.identifier.otherWOS: 000884346100004
dc.identifier.otherORCID: /0000-0001-7590-3042/work/123728331
dc.identifier.urihttp://hdl.handle.net/10362/145834
dc.identifier.urlhttps://www.scopus.com/pages/publications/85131968606
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FNAN-OPT%2F31596%2F2017/PT
dc.subjectAtomic Force Microscopy (AFM)
dc.subjectFinite Element Modelling (FEM)
dc.subjectFinite-Difference Time Domain (FDTD)
dc.subjectFluorescence Microscopy
dc.subjectHybrid AFM-Fluorescence Microscopy
dc.subjectPhotothermal excitation
dc.subjectGeneral Physics and Astronomy
dc.titlePredicting sample heating induced by cantilevers illuminated by intense light beamsen
dc.typejournal article
degois.publication.titleResults in Physics
degois.publication.volume39
dspace.entity.typePublication
oaire.awardNumberPTDC/NAN-OPT/31596/2017
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FNAN-OPT%2F31596%2F2017/PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
relation.isProjectOfPublication3b859b87-d1af-4a7c-8681-45f1d4408715
relation.isProjectOfPublication.latestForDiscovery3b859b87-d1af-4a7c-8681-45f1d4408715

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