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Microelectrode sensor for real-time measurements of nitrite in the living brain, in the presence of ascorbate

2021-08Artigo científico Acesso aberto
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dc.contributor.authorMonteiro, Tiago
dc.contributor.authorDias, Cândida
dc.contributor.authorLourenço, Cátia F.
dc.contributor.authorLedo, Ana
dc.contributor.authorBarbosa, Rui M.
dc.contributor.authorAlmeida, M. Gabriela
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2022-12-02T22:14:56Z
dc.date.available2022-12-02T22:14:56Z
dc.date.issued2021-08
dc.description
dc.description.abstractThe impaired blood flow to the brain causes a decrease in the supply of oxygen that can result in cerebral ischemia; if the blood flow is not restored quickly, neuronal injury or death will occur. Under hypoxic conditions, the production of nitric oxide (• NO), via the classical L-arginine–• NO synthase pathway, is reduced, which can compromise• NO-dependent vasodilation. However, the alternative nitrite (NO2− ) reduction to• NO, under neuronal hypoxia and ischemia conditions, has been viewed as an in vivo storage pool of• NO, complementing its enzymatic synthesis. Brain research is thus demanding suitable tools to probe nitrite’s temporal and spatial dynamics in vivo. In this work, we propose a new method for the real-time measurement of nitrite concentration in the brain extracellular space, using fast-scan cyclic voltammetry (FSCV) and carbon microfiber electrodes as sensing probes. In this way, nitrite was detected anodically and in vitro, in the 5–500 µM range, in the presence of increasing physiological concentrations of ascorbate (100–500 µM). These sensors were then tested for real-time and in vivo recordings in the anesthetized rat hippocampus; using fast electrochemical techniques, local and reproducible transients of nitrite oxidation signals were observed, upon pressure ejection of an exogenous nitrite solution into the brain tissue. Nitrite microsensors are thus a valuable tool for investigating the role of this inorganic anion in brain redox signaling.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent12
dc.format.extent1974809
dc.identifier.doi10.3390/bios11080277
dc.identifier.issn2079-6374
dc.identifier.otherPURE: 45662895
dc.identifier.otherPURE UUID: ec1d426e-c611-42fa-8c2e-b0df8b14fb96
dc.identifier.otherScopus: 85113518945
dc.identifier.otherPubMed: 34436079
dc.identifier.otherWOS: 000688918400001
dc.identifier.otherPubMedCentral: PMC8394717
dc.identifier.urihttp://hdl.handle.net/10362/145966
dc.identifier.urlhttps://www.scopus.com/pages/publications/85113518945
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/OE/PD%2FBD%2F109687%2F2015/PT
dc.relationNovos biomarcadores de doenças cardiovasculares: deteção rápida de Homoscisteína-tiolactona e implicações bioquímicas em amostras de sangue humano e no diagnóstico clínico
dc.subjectCarbon fiber microelectrodes
dc.subjectFast-scan cyclic voltammetry
dc.subjectHippocampus
dc.subjectNitrite
dc.subjectAnalytical Chemistry
dc.subjectBiotechnology
dc.subjectBiomedical Engineering
dc.subjectInstrumentation
dc.subjectEngineering (miscellaneous)
dc.subjectClinical Biochemistry
dc.titleMicroelectrode sensor for real-time measurements of nitrite in the living brain, in the presence of ascorbateen
dc.typejournal article
degois.publication.issue8
degois.publication.titleBiosensors
degois.publication.volume11
dspace.entity.typePublication
oaire.awardNumberPD/BD/109687/2015
oaire.awardTitleNovos biomarcadores de doenças cardiovasculares: deteção rápida de Homoscisteína-tiolactona e implicações bioquímicas em amostras de sangue humano e no diagnóstico clínico
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/PD%2FBD%2F109687%2F2015/PT
oaire.fundingStreamOE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
relation.isProjectOfPublicationd6e9ccaf-5644-449c-b961-2c67d31b65da
relation.isProjectOfPublication.latestForDiscoveryd6e9ccaf-5644-449c-b961-2c67d31b65da

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