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H-2 enrichment distribution of hepatic glycogen from (H2O)-H-2 reveals the contribution of dietary fructose to glycogen synthesis

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dc.contributor.authorMacedo, MP
dc.contributor.authorMartins, Fátima Oliveira
dc.contributor.authorDelgado, Teresa
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.institutionCentro de Estudos de Doenças Crónicas (CEDOC)
dc.contributor.pblAmerican Physiological Society
dc.date.accessioned2023-12-28T22:07:04Z
dc.date.available2023-12-28T22:07:04Z
dc.date.issued2013
dc.description.abstractDelgado TC, Martins FO, Carvalho F, Goncalves A, Scott DK, O'Doherty R, Macedo MP, Jones JG. H-2 enrichment distribution of hepatic glycogen from (H2O)-H-2 reveals the contribution of dietary fructose to glycogen synthesis. Am J Physiol Endocrinol Metab 304: E384-E391, 2013. First published December 4, 2012; doi:10.1152/ajpendo.00185.2012.-Dietary fructose can benefit or hinder glycemic control, depending on the quantity consumed, and these contrasting effects are reflected by alterations in postprandial hepatic glycogen synthesis. Recently, we showed that H-2 enrichment of glycogen positions 5 and 2 from deuterated water ((H2O)-H-2) informs direct and indirect pathway contributions to glycogenesis in naturally feeding rats. Inclusion of position 6(S) H-2 enrichment data allows indirect pathway sources to be further resolved into triose phosphate and Krebs cycle precursors. This analysis was applied to six rats that had fed on standard chow (SC) and six rats that had fed on SC plus 35\% sucrose in their drinking water (HS). After 2 wk, hepatic glycogenesis sources during overnight feeding were determined by (H2O)-H-2 administration and postmortem analysis of glycogen H-2 enrichment at the conclusion of the dark period. Net overnight hepatic glycogenesis was similar between SC and HS rodents. Whereas direct pathway contributions were similar (403 +/- 71 mu mol/g dry wt HS vs. 578 +/- 76 mu mol/g dry wt SC), triose phosphate contributions were significantly higher for HS compared with SC (382 +/- 61 vs. 87 +/- 24 mu mol/g dry wt, P < 0.01) and Krebs cycle inputs lower for HS compared with SC (110 +/- 9 vs. 197 +/- 32 mu mol/g dry wt, P < 0.05). Analysis of plasma glucose H-2 enrichments at the end of the feeding period also revealed a significantly higher fractional contribution of triose phosphate to plasma glucose levels in HS vs. SC. Hence, the H-2 enrichment distributions of hepatic glycogen and glucose from (H2O)-H-2 inform the contribution of dietary fructose to hepatic glycogen and glucose synthesis.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent204454
dc.identifier.doi10.1152/ajpendo.00185.2012
dc.identifier.issn0193-1849
dc.identifier.otherPURE: 309194
dc.identifier.otherPURE UUID: e9d8f8fc-6229-41e5-82f9-6eef4e233db6
dc.identifier.otherresearchoutputwizard: 37530
dc.identifier.otherPubMed: 23211519
dc.identifier.otherWOS: 000315141900006
dc.identifier.otherScopus: 84874073240
dc.identifier.otherORCID: /0000-0002-2549-0275/work/151416100
dc.identifier.urihttp://hdl.handle.net/10362/161698
dc.language.isoeng
dc.peerreviewedyes
dc.subjecthepatic glycogen
dc.subjectINSULIN-RESISTANCE
dc.subjectdeuterated water
dc.subjectsucrose
dc.subjectORAL GLUCOSE-TOLERANCE
dc.subjectRAT-LIVER
dc.subjectTRANSALDOLASE EXCHANGE
dc.subjectdirect and indirect pathways
dc.subjectGLUCONEOGENESIS
dc.subjectMETABOLISM
dc.subjectfructose
dc.subjectINGESTION
dc.subjectNMR
dc.subjectSUCROSE
dc.subjectLIPOPROTEIN-LIPASE
dc.titleH-2 enrichment distribution of hepatic glycogen from (H2O)-H-2 reveals the contribution of dietary fructose to glycogen synthesisen
dc.typejournal article
degois.publication.firstPageE384
degois.publication.issue4
degois.publication.lastPageE391
degois.publication.titleAmerican Journal Of Physiology-Endocrinology And Metabolism
degois.publication.volume304
dspace.entity.typePublication
rcaap.rightsopenAccess

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