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Occluding junctions as novel regulators of tissue mechanics during wound repair

2018-12-01Artigo científico Acesso aberto
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dc.contributor.authorCarvalho, Lara
dc.contributor.authorPatricio, Pedro
dc.contributor.authorPonte, Susana
dc.contributor.authorHeisenberg, Carl Philipp
dc.contributor.authorAlmeida, Luis
dc.contributor.authorNunes, André S.
dc.contributor.authorAraújo, Nuno A.M.
dc.contributor.authorJacinto, Antonio
dc.contributor.authorJacinto, Antonio
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.institutionCentro de Estudos de Doenças Crónicas (CEDOC)
dc.contributor.pblRockefeller University Press
dc.date.accessioned2022-10-28T22:05:24Z
dc.date.available2022-10-28T22:05:24Z
dc.date.issued2018-12-01
dc.descriptionThis research was supported by Fundação para a Ciência e a Tecnologia (SFRH/BPD/84569/2012 to L. Carvalho, PD/ BD/106058/2015 to S. Ponte, SFRH/BD/119240/2016 to A.S. Nunes, UID/FIS/00618/2013, PTDC/FIS-MAC/28146/2017, PTDC/ BIA-BID/29709/2017 and CONGENTO), the European Research Council (2007-StG-208631 and ERC-2015-PoC-713735-EMODI), a European Commission Marie Curie Intra-European Fellowship (PIEF-GA-2009-255573), the Partenariat Hubert Curien Pessoa program (30920XM), the European Science Foundation (Quantitative Models of Cellular and Developmental Biology Research Networking Program, grant 6839), and the iNOVA4Health Research Unit (UID/Multi/04462/2013), cofunded by Fundação para a Ciência e Tecnologia, through national funds and by FED ER under the PT2020 Partnership Agreement.
dc.description.abstractIn epithelial tissues, cells tightly connect to each other through cell–cell junctions, but they also present the remarkable capacity of reorganizing themselves without compromising tissue integrity. Upon injury, simple epithelia efficiently resolve small lesions through the action of actin cytoskeleton contractile structures at the wound edge and cellular rearrangements. However, the underlying mechanisms and how they cooperate are still poorly understood. In this study, we combine live imaging and theoretical modeling to reveal a novel and indispensable role for occluding junctions (OJs) in this process. We demonstrate that OJ loss of function leads to defects in wound-closure dynamics: instead of contracting, wounds dramatically increase their area. OJ mutants exhibit phenotypes in cell shape, cellular rearrangements, and mechanical properties as well as in actin cytoskeleton dynamics at the wound edge. We propose that OJs are essential for wound closure by impacting on epithelial mechanics at the tissue level, which in turn is crucial for correct regulation of the cellular events occurring at the wound edge.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent17
dc.format.extent3982311
dc.identifier.doi10.1083/jcb.201804048
dc.identifier.issn0021-9525
dc.identifier.otherPURE: 10673705
dc.identifier.otherPURE UUID: cd5cdd64-f428-4484-8a1a-4d869c1df822
dc.identifier.otherScopus: 85057996130
dc.identifier.otherPubMed: 30228162
dc.identifier.otherWOS: 000451960800018
dc.identifier.otherORCID: /0000-0002-4193-6089/work/51947750
dc.identifier.urihttp://hdl.handle.net/10362/145094
dc.identifier.urlhttps://www.scopus.com/pages/publications/85057996130
dc.language.isoeng
dc.peerreviewedyes
dc.subjectCell Biology
dc.titleOccluding junctions as novel regulators of tissue mechanics during wound repairen
dc.typejournal article
degois.publication.firstPage4267
degois.publication.issue12
degois.publication.lastPage4283
degois.publication.titleJournal of Cell Biology
degois.publication.volume217
dspace.entity.typePublication
person.familyNameJacinto
person.givenNameAntonio
person.identifier.ciencia-idDF15-1DEB-A48C
person.identifier.orcid0000-0002-4193-6089
person.identifier.ridF-5729-2013
person.identifier.scopus-author-id55880355900
rcaap.rightsopenAccess
relation.isAuthorOfPublication37ff3a19-e7d7-4c9f-af75-fade2f9aa9d1
relation.isAuthorOfPublication.latestForDiscovery37ff3a19-e7d7-4c9f-af75-fade2f9aa9d1

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