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Dand5 is involved in zebrafish tailbud cell movement

2023-01-09Artigo científico Acesso aberto
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dc.contributor.authorBota, Catarina
dc.contributor.authorMartins, Gabriel G.
dc.contributor.authorLopes, Susana S.
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.institutioniNOVA4Health - pólo NMS
dc.contributor.pblFrontiers Media
dc.date.accessioned2023-02-14T22:21:41Z
dc.date.available2023-02-14T22:21:41Z
dc.date.issued2023-01-09
dc.descriptionFunding Information: This work was supported by the Fundação para a Ciência e a Tecnologia (FCT-ANR/BEX-BID/0153/2012 research grant and by the Project LysoCil funded by the European Union Horizon 2020 research and innovation under grant agreement No. 811087. SSL was funded by an FCT CEEC contract for Principal Investigator reference 2018CEECIND/02170/2018 and CB was funded by a FCT SFRH/BD/141034/2018 PhD fellowship. NMS and IGC Fish Facilities were supported from the research infrastructure Congento, co-financed by Lisboa Regional Operational Programme (Lisboa 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) and FCT under the project LISBOA-01-0145-FEDER-022170. Publisher Copyright: Copyright © 2023 Bota, Martins and Lopes.
dc.description.abstractDuring vertebrate development, symmetry breaking occurs in the left-right organizer (LRO). The transfer of asymmetric molecular information to the lateral plate mesoderm is essential for the precise patterning of asymmetric internal organs, such as the heart. However, at the same developmental time, it is crucial to maintain symmetry at the somite level for correct musculature and vertebrae specification. We demonstrate how left-right signals affect the behavior of zebrafish somite cell precursors by using live imaging and fate mapping studies in dand5 homozygous mutants compared to wildtype embryos. We describe a population of cells in the vicinity of the LRO, named Non-KV Sox17:GFP+ Tailbud Cells (NKSTCs), which migrate anteriorly and contribute to future somites. We show that NKSTCs originate in a cluster of cells aligned with the midline, posterior to the LRO, and leave that cluster in a left-right alternating manner, primarily from the left side. Fate mapping revealed that more NKSTCs integrated somites on the left side of the embryo. We then abolished the asymmetric cues from the LRO using dand5−/− mutant embryos and verified that NKSTCs no longer displayed asymmetric patterns. Cell exit from the posterior cluster became bilaterally synchronous in dand5−/− mutants. Our study revealed a new link between somite specification and Dand5 function. The gene dand5 is well known as the first asymmetric gene involved in vertebrate LR development. This study revealed a new link for Dand5 as a player in cell exit from the maturation zone into the presomitic mesoderm, affecting the expression patterns of myogenic factors and tail size.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent4502809
dc.identifier.doi10.3389/fcell.2022.989615
dc.identifier.issn2296-634X
dc.identifier.otherPURE: 52556710
dc.identifier.otherPURE UUID: 6fcd9638-17a8-4e8d-8fe2-84afb5a19a8d
dc.identifier.otherScopus: 85146907344
dc.identifier.otherPubMed: 36699016
dc.identifier.urihttp://hdl.handle.net/10362/149217
dc.identifier.urlhttps://www.scopus.com/pages/publications/85146907344
dc.language.isoeng
dc.peerreviewedyes
dc.subjectasymmetry
dc.subjectcell migration
dc.subjectDand5
dc.subjectleft-right development
dc.subjectpre-somitic mesoderm
dc.subjectsomites
dc.subjectsymmetry
dc.subjectDevelopmental Biology
dc.subjectCell Biology
dc.titleDand5 is involved in zebrafish tailbud cell movementen
dc.typejournal article
degois.publication.titleFrontiers in Cell and Developmental Biology
degois.publication.volume10
dspace.entity.typePublication
rcaap.rightsopenAccess

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