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A new fluorescence-based approach for direct visualization of coat formation during sporulation in Bacillus cereus

2023-12Artigo científico Acesso aberto
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dc.contributor.authorLablaine, Armand
dc.contributor.authorChamot, Stéphanie
dc.contributor.authorSerrano, Mónica
dc.contributor.authorBillaudeau, Cyrille
dc.contributor.authorBornard, Isabelle
dc.contributor.authorCarballido-López, Rut
dc.contributor.authorCarlin, Frédéric
dc.contributor.authorHenriques, Adriano O.
dc.contributor.authorBroussolle, Véronique
dc.contributor.institutionInstituto de Tecnologia Química e Biológica António Xavier (ITQB)
dc.contributor.pblNature Publishing Group
dc.date.accessioned2024-04-02T23:51:50Z
dc.date.available2024-04-02T23:51:50Z
dc.date.issued2023-12
dc.descriptionFunding Information: The Ph.D. thesis of A.L. was funded by INRAE and the PACA Region and was partly supported by a grant of the MICA division and a Perdiguier grant of Avignon University. Part of this work was supported by the microscopy facilities of the Platform 3A, funded by the European Regional Development Fund, the French Ministry of Research, Higher Education and Innovation, the Provence-Alpes-Côte d’Azur region, the Departmental Council of Vaucluse and the Urban Community of Avignon. This work was also funded through grants PEst-OE/EQB/LA0004/2011 to AOH, by project LISBOA-01-0145-FEDER-007660 (“Microbiologia Molecular, Estrutural e Celular”) funded by FEDER funds through COMPETE2020 – “Programa Operacional Competitividade e Internacionalização”, and by project PPBI—Portuguese Platform of BioImaging (PPBI-POCI-01-0145-FEDER-022122) co-funded by national funds from OE—"Orçamento de Estado" and by European funds from FEDER—"Fundo Europeu de Desenvolvimento Regional". Work and Lattice SIM imaging in the R.C-L. lab was supported by funding from the European Research Council (ERC) under the Horizon 2020 research and innovation program (grant agreement No 772178, ERC Consolidator grant to R.C.-L.). Publisher Copyright: © 2023, Springer Nature Limited.
dc.description.abstractThe human pathogenic bacteria Bacillus cereus, Bacillus anthracis and the entomopathogenic Bacillus thuringiensis form spores encased in a protein coat surrounded by a balloon-like exosporium. These structures mediate spore interactions with its environment, including the host immune system, control the transit of molecules that trigger germination and thus are essential for the spore life cycle. Formation of the coat and exosporium has been traditionally visualized by transmission electronic microscopy on fixed cells. Recently, we showed that assembly of the exosporium can be directly observed in live B. cereus cells by super resolution-structured illumination microscopy (SR-SIM) using the membrane MitoTrackerGreen (MTG) dye. Here, we demonstrate that the different steps of coat formation can also be visualized by SR-SIM using MTG and SNAP-cell TMR-star dyes during B. cereus sporulation. We used these markers to characterize a subpopulation of engulfment-defective B. cereus cells that develops at a suboptimal sporulation temperature. Importantly, we predicted and confirmed that synthesis and accumulation of coat material, as well as synthesis of the σK-dependent protein BxpB, occur in cells arrested during engulfment. These results suggest that, unlike the well-studied model organism Bacillus subtilis, the activity of σK is not strictly linked to the state of forespore development in B. cereus.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent7959332
dc.identifier.doi10.1038/s41598-023-42143-9
dc.identifier.issn2045-2322
dc.identifier.otherPURE: 83700875
dc.identifier.otherPURE UUID: 21f20444-83c1-4d5b-aa4c-23b5287d41ac
dc.identifier.otherScopus: 85171141169
dc.identifier.otherPubMed: 37704668
dc.identifier.urihttp://hdl.handle.net/10362/165738
dc.identifier.urlhttps://www.scopus.com/pages/publications/85171141169
dc.language.isoeng
dc.peerreviewedyes
dc.subjectGeneral
dc.titleA new fluorescence-based approach for direct visualization of coat formation during sporulation in Bacillus cereusen
dc.typejournal article
degois.publication.issue1
degois.publication.titleScientific Reports
degois.publication.volume13
dspace.entity.typePublication
rcaap.rightsopenAccess

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