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Differential incidence of tongue base cancer in male and female hpv16-transgenic mice

2021-10Artigo científico Acesso aberto
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dc.contributor.authorNeto, Clariano Pires de Oliveira
dc.contributor.authorMedeiros-Fonseca, Beatriz
dc.contributor.authorEstêvão, Diogo
dc.contributor.authorMestre, Verónica F.
dc.contributor.authorCosta, Natália R.
dc.contributor.authorde Andrade, Fábio Evangelista
dc.contributor.authorOliveira, Paula A.
dc.contributor.authorBastos, Margarida M.S.M.
dc.contributor.authorMedeiros, Rui
dc.contributor.authorAssis, Diogo
dc.contributor.authorFélix, Ana
dc.contributor.authorLopes, Fernanda Ferreira
dc.contributor.authorGil da Costa, Rui M.
dc.contributor.authorBrito, Haissa O.
dc.contributor.authorBrito, Luciane M.O.
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2021-10-22T03:43:05Z
dc.date.available2021-10-22T03:43:05Z
dc.date.issued2021-10
dc.descriptionFunding Information: Funding: This study was supported by grant IECT-FAPEMA-05796/18 and FAPEMA IECT 30/2018-IECT Saúde, by the Research Center of the Portuguese Oncology Institute of Porto (project no. PI86-CI-IPOP-66-2017); by European Investment Funds by FEDER/COMPETE/POCI—Operational Competitiveness and Internationalization Program, and national funds by FCT—Portuguese Foundation for Science and Technology under projects UID/AGR/04033/2020, UIDB/CVT/00772/2020 and by Base Funding-UIDB/00511/2020 of the Laboratory for Process Engineering, Environment, Biotechnology, and Energy—LEPABE—funded by national funds through the FCT/MCTES (PID-DAC); Project 2SMART-engineered Smart materials for Smart citizens, with reference NORTE-01-0145-FEDER-000054, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
dc.description.abstractA growing proportion of oropharyngeal squamous cell carcinomas (OPSCC) are associated with infection by high-risk human papillomavirus (HPV). For reasons that remain largely unknown, HPV+OPSCC is significantly more common in men than in women. This study aims to determine the incidence of OPSCC in male and female HPV16-transgenic mice and to explore the role of female sex hormone receptors in the sexual predisposition for HPV+ OPSCC. The tongues of 30-weeks-old HPV16-transgenic male (n = 80) and female (n = 90) and matched wild-type male (n = 10) and female (n = 10) FVB/n mice were screened histologically for intraepithelial and invasive lesions in 2017 at the Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Por-tugal. Expression of estrogen receptors alpha (ERα) and beta (ERβ), progesterone receptors (PR) and matrix metalloproteinase 2 (MMP2) was studied immunohistochemically. Collagen remodeling was studied using picrosirius red. Female mice showed robust ERα and ERβ expression in intraepithelial and invasive lesions, which was accompanied by strong MMP2 expression and marked collagen remodeling. Male mice showed minimal ERα, ERβ and MMP2 expression and unaltered collagen patterns. These results confirm the association of HPV16 with tongue base cancer in both sexes. The higher cancer incidence in female versus male mice contrasts with data from OPSCC patients and is associated with enhanced ER expression via MMP2 upregulation.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent3398784
dc.identifier.doi10.3390/pathogens10101224
dc.identifier.issn2076-0817
dc.identifier.otherPURE: 34091043
dc.identifier.otherPURE UUID: 0d43ca30-dbbe-466c-b7ef-aeec1e813418
dc.identifier.otherScopus: 85115877525
dc.identifier.otherORCID: /0000-0002-2653-2262/work/101793982
dc.identifier.otherPubMed: 34684173
dc.identifier.otherPubMedCentral: PMC8539196
dc.identifier.urihttp://hdl.handle.net/10362/126481
dc.identifier.urlhttps://www.scopus.com/pages/publications/85115877525
dc.language.isoeng
dc.peerreviewedyes
dc.subjectCollagen
dc.subjectEstrogen receptor
dc.subjectMatrix metalloproteinase
dc.subjectProgesterone receptor
dc.subjectTongue base cancer
dc.subjectImmunology and Allergy
dc.subjectMolecular Biology
dc.subjectGeneral Immunology and Microbiology
dc.subjectMicrobiology (medical)
dc.subjectInfectious Diseases
dc.subjectSDG 3 - Good Health and Well-being
dc.titleDifferential incidence of tongue base cancer in male and female hpv16-transgenic miceen
dc.title.subtitlerole of female sex hormone receptorsen
dc.typejournal article
degois.publication.issue10
degois.publication.titlePathogens
degois.publication.volume10
dspace.entity.typePublication
rcaap.rightsopenAccess

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