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Cytocompatibility, fibroblast adhesion and proliferation on surface modified 3D-printed PEEK scaffolds

2025-07Artigo científico Acesso aberto
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dc.contributor.authorRendas, Pedro
dc.contributor.authorAmorim, Joana
dc.contributor.authorBaptista, Pedro Viana
dc.contributor.authorVidal, Catarina
dc.contributor.authorFigueiredo, Lígia
dc.contributor.authorFernandes, Alexandra R.
dc.contributor.authorSoares, Bruno
dc.contributor.institutionDEMI - Departamento de Engenharia Mecânica e Industrial
dc.contributor.institutionUNIDEMI - Unidade de Investigação e Desenvolvimento em Engenharia Mecânica e Industrial
dc.contributor.institutionDCV - Departamento de Ciências da Vida
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.pblElsevier
dc.date.accessioned2025-07-21T21:13:39Z
dc.date.available2025-07-21T21:13:39Z
dc.date.issued2025-07
dc.descriptionFunding Information: The authors acknowledge Fundação para a Ciência e a Tecnologia (FCT-MCTES) for its financial support through the PhD scholarship UI/BD/151082/2021, through the projects UIDB/00667/2020 and UIDP/00667/2020 of UNIDEMI, the projects UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences – UCIBIO, the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy - i4HB and the project NANOHEAT (2022.04315.PTDC). The authors would also like to thank Professor João Sottomayor (LAQV, Department of Chemistry, NOVA School of Science and Technology) for access to the wettability measuring equipment. Publisher Copyright: © 2025 The Authors
dc.description.abstractPolyetheretherketone (PEEK) is a high-performance thermoplastic that, when combined with Additive Manufacturing (AM), presents considerable advantages to produce customizable implantable medical devices. Despite this potential, PEEK's use as an implant material still presents challenges imposed by its bioinert nature. This study investigates the biofunctionalization of 3D-printed PEEK implants to enhance fibroblasts' cellular response due to their important role in the healing of connective tissue post-implantation. Different combinations of biofunctional features were investigated by surface modifying solid, porous, and surface-rough 3D-printed PEEK samples with the sulfonation treatment and incorporation of hydroxyapatite (HA) particles. The porous scaffold construct was designed based on a gyroid surface and then analysed using micro-CT and compression tests. Fibroblast culture assays were conducted to assess the effects of different surface morphologies on cellular adhesion and proliferation. Preliminary data of fibroblast metabolic activity on differently modified PEEK samples was also collected. Results from the experiments suggest that solid PEEK samples with rough surfaces and subjected to both sulfonation and HA incorporation procedures exhibit the most favourable environment for maintaining fibroblasts morphology and viability. Conversely, the lower adhesion and proliferation on smooth as-printed surfaces highlight the necessity for surface functionalization of 3D-printed PEEK. Additionally, results for metabolic activity paired with cell morphologies observed under SEM indicate that large-scale porous scaffolds may present less favourable environment for fibroblasts viability compared to solid surfaces. These findings offer valuable insights to 3D-printed PEEK biofunctionalization towards the improvement of fibroblast response, particularly considering their active role on extracellular matrix formation which is critical for connective tissue support and cohesion during the healing process after surgical implantation.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent13
dc.format.extent7911844
dc.identifier.doi10.1016/j.jmbbm.2025.106979
dc.identifier.issn1751-6161
dc.identifier.otherPURE: 114188238
dc.identifier.otherPURE UUID: b66cac0a-6a09-4940-8f9b-7d91a00483b5
dc.identifier.otherScopus: 105000269295
dc.identifier.otherWOS: 001450759700001
dc.identifier.otherPubMed: 40112483
dc.identifier.otherORCID: /0000-0002-7622-847X/work/188369238
dc.identifier.otherORCID: /0000-0001-5255-7095/work/188369293
dc.identifier.otherORCID: /0000-0003-2737-1154/work/188369665
dc.identifier.otherORCID: /0000-0003-2054-4438/work/188369926
dc.identifier.urihttp://hdl.handle.net/10362/185416
dc.identifier.urlhttps://www.scopus.com/pages/publications/105000269295
dc.identifier.urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=nova_api&SrcAuth=WosAPI&KeyUT=WOS:001450759700001&DestLinkType=FullRecord&DestApp=WOS_CPL
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/OE/UI%2FBD%2F151082%2F2021/PT
dc.relationDesenvolvimento duma nova geração de próteses baseadas em impressão 3D de PEEK com reforço bio cerâmico através de processamento no estado sólido provisório
dc.relationResearch and Development Unit for Mechanical and Industrial Engineering
dc.relationResearch and Development Unit for Mechanical and Industrial Engineering
dc.relationApplied Molecular Biosciences Unit
dc.relationApplied Molecular Biosciences Unit
dc.relationNANOHEAT – Gold nanoconjugates for improved photothermal ablation of colorectal cancer
dc.relationinfo:eu-repo/grantAgreement/FCT/Concurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)/LA%2FP%2F0140%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/2022.04315.PTDC/PT
dc.subjectAdditive manufacturing (AM)
dc.subjectFibroblasts
dc.subjectPolyetheretherketone (PEEK)
dc.subjectScaffold
dc.subjectSurface modification
dc.subjectBiomaterials
dc.subjectBiomedical Engineering
dc.subjectMechanics of Materials
dc.titleCytocompatibility, fibroblast adhesion and proliferation on surface modified 3D-printed PEEK scaffoldsen
dc.typejournal article
degois.publication.firstPage1
degois.publication.lastPage13
degois.publication.titleJournal of the Mechanical Behavior of Biomedical Materials
degois.publication.volume167
dspace.entity.typePublication
oaire.awardNumberUI/BD/151082/2021
oaire.awardNumberUIDB/00667/2020
oaire.awardNumberUIDP/00667/2020
oaire.awardNumberUIDP/04378/2020
oaire.awardNumberUIDB/04378/2020
oaire.awardNumberLA/P/0140/2020
oaire.awardNumber2022.04315.PTDC
oaire.awardTitleDesenvolvimento duma nova geração de próteses baseadas em impressão 3D de PEEK com reforço bio cerâmico através de processamento no estado sólido provisório
oaire.awardTitleResearch and Development Unit for Mechanical and Industrial Engineering
oaire.awardTitleResearch and Development Unit for Mechanical and Industrial Engineering
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleNANOHEAT – Gold nanoconjugates for improved photothermal ablation of colorectal cancer
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/UI%2FBD%2F151082%2F2021/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00667%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00667%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)/LA%2FP%2F0140%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/2022.04315.PTDC/PT
oaire.fundingStreamOE
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamConcurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
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