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Projeto de investigação
iSkin2: improving skin regeneration through an improved Skin2 biosynthetic skin substitute
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Electrospun Polycaprolactone Membranes Expanded with Chitosan Granules for Cell Infiltration
Publication . Vieira, Tânia; Rebelo, Ana Margarida; Borges, João Paulo; Henriques, Célia; Silva, Jorge Carvalho; DF – Departamento de Física; CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N); DCM - Departamento de Ciência dos Materiais; MDPI - Multidisciplinary Digital Publishing Institute
The small pore size of electrospun membranes prevents their use as three-dimensional scaffolds. In this work, we produced polycaprolactone (PCL) electrospun fibrous membranes with expanded pores by incorporating chitosan (CS) granules into the PCL solution. Scanning electron microscopy images confirmed the presence of the CS granules embedded in the PCL fibers, creating an open structure. Tensile testing results showed that the addition of CS decreased both Young’s modulus and the yield stress, but co-electrospun membranes (PCL fibers blended with CS-containing PCL fibers) exhibited higher values compared to single electrospun membranes (CS-containing PCL fibers). Human fibroblasts adhered to and proliferated on all scaffolds. Nuclear staining revealed that cells populated the entire scaffold when CS granules were present, while in PCL membranes, cells were mostly limited to the surface due to the small pore size. Overall, our findings demonstrate that electrospun membranes containing CS granules have sufficiently large pores to facilitate fibroblast infiltration without compromising the mechanical stability of the structure.
Electrospun poly(lactic acid) membranes with defined pore size to enhance cell infiltration
Publication . Vieira, Tânia; Afonso, Ana Filipa; Correia, Catarina; Henriques, Célia; Borges, João Paulo; Silva, Jorge Carvalho; CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N); DF – Departamento de Física; Faculdade de Ciências e Tecnologia (FCT); DCM - Departamento de Ciência dos Materiais; Elsevier
Electrospun membranes are compact structures with small pore sizes that hinder cell infiltration, resulting in membranes with cells attached only to the external surface rather than throughout the entire volume. Thus, there is a need to increase the pore size of electrospun membranes maintaining their structural similarity to the extracellular matrix. In this work, we used glucose crystals embedded in polyethylene oxide (PEO) fibers to create large pores in poly(lactic acid) (PLA) electrospun membranes to allow for cellular infiltration. The PEO fibers containing glucose crystals of different sizes (>50, 50–100 and 100–150 μm) and in varying concentrations (10, 15 and 20 %) were co-electrospun with PLA fibers and subsequently leached out using distilled water. PLA fibrous membranes without glucose crystals were also produced as controls. The membranes were examined for their morphology, mechanical properties, and potential to support the proliferation of fibroblasts. In addition, the immune response to the membranes was evaluated using monocyte-derived macrophages. The glucose crystals were uniformly distributed in the PLA membranes and their removal created open pores without collapsing the structure. Although a reduced Young's modulus was observed for membranes produced using higher glucose crystal concentrations and larger crystal sizes, the structural integrity remained intact, and the values are still suitable for tissue engineering. In vitro results showed that the scaffolds supported the adhesion and proliferation of fibroblasts and the pores created in the PLAmembranes were large enough for fibroblasts infiltration and colonization of the entire scaffold without inducing an inflammatory response.
Study on the Incorporation of Chitosan Flakes in Electrospun Polycaprolactone Scaffolds
Publication . Querido, Diana; Vieira, Tânia; Ferreira, José Luís; Henriques, Célia; Borges, João Paulo; Silva, Jorge Carvalho; DF – Departamento de Física; CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N); DCM - Departamento de Ciência dos Materiais; MDPI - Multidisciplinary Digital Publishing Institute
Hybrid scaffolds obtained by combining two or more biopolymers are studied in the context of tissue regeneration due to the possibility of achieving new functional properties or structural features. The aim of this work was to produce a new type of hybrid polycaprolactone (PCL)/chitosan (CS) electrospun mat through the controlled deposition of CS flakes interspaced between the PCL fibers. A poly(ethylene oxide) (PEO) solution was used to transport CS flakes with controlled size. This, and the PCL solution, were simultaneously electrospun onto a rotatory mandrel in a perpendicular setup. Different PCL/CS mass ratios were also studied. The morphology of the resulting fibers, evaluated by SEM, confirmed the presence of the CS flakes between the PCL fibers. The addition of PEO/CS fibers resulted in hydrophilic mats with lower Young's modulus relatively to PCL mats. In vitro cell culture results indicated that the addition of CS lowers both the adhesion and the proliferation of human dermal fibroblasts. The present work demonstrates the feasibility of achieving a controlled deposition of a polymeric component in granular form onto a collector where electrospun nanofibers are being deposited, thereby producing a hybrid scaffold.
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Entidade financiadora
Fundação para a Ciência e a Tecnologia
Programa de financiamento
9471 - RIDTI
Número da atribuição
PTDC/BTM-MAT/31470/2017