In silico models guiding in vitro biophysical stimulation of biomimetic hierarchical scaffolds: a computational modelling approach towards functional osteochondral regeneration

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Design of magnetic kappa-carrageenan-collagen bioinks for 3D bioprinting

Publication . Almeida, Duarte; Küppers, Freya; Gusmão, Afonso; Manjua, Ana C.; Ferreira, Catarina F.R.; Portugal, Carla A.M.; Silva, João C.; Sanjuan-Alberte, Paola; Ferreira, Frederico Castelo; LAQV@REQUIMTE; DQ - Departamento de Química; Springer

Bioprinting approaches are of great promise for tissue engineering applications as they allow the fabrication of constructs able to mimic native tissues’ mechanical and topographical features. Additional control over cells fate can be enhanced using stimuli-responsive materials, requiring the development of novel bioinks for this purpose. In this study, bioinks comprising κ-carrageenan, collagen, and magnetic nanoparticles were designed for 3D bioprinting applications. The characterization of this material was performed, where mechanical compressive tests yielded Young’s moduli ranging from 8.25 to 18.4 kPa. Rheological assessments also revealed the shear-thinning behavior of the bioinks and a temperature-dependent gelation. The capability of these bioinks to produce 3D constructs by extrusion bioprinting was established through the printability evaluation and the development of complex structures, supporting the viability and proliferation of mesenchymal stromal cells (MSCs). Finally, as proof-of-concept, it was observed that the secretome of bioprinted MSCs stimulated with an external magnetic field of 80 mT was able to increase the number of tubes formed by human umbilical vein endothelial cells.

2024-08Artigo científico

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Fundação para a Ciência e a Tecnologia

Programa de financiamento

3599-PPCDT

Número da atribuição

PTDC/EME-SIS/0838/2021