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Unveiling the anti-inflammatory potential of 11β,13-dihydrolactucin for application in inflammatory bowel disease management
Publication . Matos, Melanie S.; Avila-Galvez, Maria Angeles; Gonzalez-Sarrias, Antonio; Silva, Nuno Valerio; Crespo, Carolina Lage; Jacinto, Antonio; Serra, Ana Teresa; Matias, Ana A.; Nunes dos Santos, Claudia; Instituto de Tecnologia Química e Biológica António Xavier (ITQB); NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM); NOVA Institute for Medical Systems Biology; RSC - Royal Society of Chemistry
Management of inflammatory bowel disease (IBD) poses significant challenges, and there is a need for innovative therapeutic approaches. This study investigates the anti-inflammatory properties of the dietary sesquiterpene lactone (SL) 11β,13-dihydrolactucin, which can be found in chicory, in three distinct complementary models of intestinal inflammation (two cell models and a zebrafish model), offering comprehensive insights into its potential application for IBD treatment alternatives. In a triple cell co-culture composed of Caco-2, HT29-MTX-E12, and Raji B, 11β,13-dihydrolactucin demonstrated remarkable anti-inflammatory activity at several levels of the cellular inflammatory response. Notably, 11β,13-dihydrolactucin prevented the activation of critical signalling pathways associated with inflammation, namely NF-κB and MAPK p38. This SL also decreased the release of the neutrophil-recruiting chemokine IL-8. Additionally, the compound reduced the gene expression of IL-6 and TNF-α, as well as the gene and protein expression of the inflammatory inducible enzymes iNOS and COX-2. In a myofibroblast-like human cell model, 11β,13-dihydrolactucin decreased the release of the cytokine TNF-α and the COX-2-derived inflammation mediator PGE2. Finally, in a zebrafish model of gut inflammation, 11β,13-dihydrolactucin effectively reduced neutrophil infiltration, further supporting its anti-inflammatory efficacy in a physiological context. Collectively, our findings highlight the promising anti-inflammatory potential of 11β,13-dihydrolactucin across various facets of intestinal inflammation, providing a foundation for the consideration of chicory as a promising candidate for incorporation in food or nutraceutical products for the potential prevention of IBD.
Adding value to chicory roots: Evaluation of the anti-inflammatory potential of terpenes for application in Inflammatory Bowel Disease
Publication . Matos, Melanie S.; Matias, Ana; Santos, Cláudia; Serra, Ana Teresa
"Inflammatoryboweldisease(IBD)isachronicgastrointestinalconditionaffecting
nearly 5millionindividualsglobally,posingasubstantialpublichealthchallenge.IBD
is amultifactorialdisorder,involvinganintricateinterplayamongtheimmunesystem,
environmentalfactors,geneticsusceptibility,andalterationsingutmicrobiota.Upto
date,thereisnocureforIBD,andcurrenttherapeuticstrategieshaveinherentlimi-
tations.Apartfromtheirhighcosts,thesesymptomatictreatmentsexhibitsideeffects
and oftenexperiencediminishedefficacyovertime,leadingtoarecurrentpatternofre-
lapseandremission.GiventhemultifacetednatureofIBDanditsindividualvariability,
thereisanurgentdemandforthediscoveryofinnovativeandeffectivepharmacological
leads, toaddresstheshortcomingsassociatedwithexistingtherapies.(...)"
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SFRH/BD/145551/2019