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Conversion of tomato waste into a poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) by a mixed microbial community
Publication . Rodrigues, Thomas; Torres, Cristiana V.; Freitas, Patrícia; Neves, Luísa A.; Carvalheira, Mónica; Reis, Maria A.M.; Freitas, Filomena; Faculdade de Ciências e Tecnologia (FCT); UCIBIO - Applied Molecular Biosciences Unit; DQ - Departamento de Química; LAQV@REQUIMTE; Elsevier BV
Tomato waste (TW) originating from tomato processing is produced in large quantities and has a low cost, presenting as an interesting feedstock for biotechnological valorization into value-added bioproducts. This study explored the utilization of TW as feedstock to produce polyhydroxyalkanoates (PHA) by a mixed microbial community. The bioprocess encompassed acidogenic fermentation of TW with a maximum yield of 0.22 ± 0.05 gFP/gVS. A maximum PHA content of 66.8 ± 11.0 wt.% was reached in the accumulation assays, with a yield of 0.75 ± 0.10 CmmolPHA/CmmolFP. The biopolymer was composed of 3-hydroxybutyrate (3HB), hydroxyvalerate (3HV) and hydroxyhexanoate (3HHx) with an average composition of 82:11:7 (3HB:3HV:3HHx, molar basis) and similar mechanical and thermal properties to other 3HB:3HV:3HHx terpolymers and comparable to high density polyethylene and polypropylene. These findings demonstrated that TW can be used to efficiently produce a PHA terpolymer with properties that render it suitable for replacing petrochemical-derived plastics.

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Fundação para a Ciência e a Tecnologia

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Concurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (info:eu-repo

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