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Projeto de investigação
Impact of mushroom nutrition on gut microbiota modulation and association with neuroprotective capacity
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Organoleptic Chemical Markers of Serpa PDO Cheese Specificity
Publication . Araújo-Rodrigues, Helena; Martins, António P. L.; Tavaria, Freni K.; Santos, Maria Teresa G.; Carvalho, Maria João; Dias, João; Alvarenga, Nuno B.; Pintado, Manuela E.; GeoBioTec - Geobiociências, Geoengenharias e Geotecnologias; MDPI - Multidisciplinary Digital Publishing Institute
Serpa is a protected designation of origin cheese produced with a vegetable coagulant (Cynara cardunculus L.) and raw ovine milk. Despite the unique sensory profile of raw milk cheeses, numerous parameters influence their sensory properties and safety. To protect the Serpa cheese quality and contribute to unifying their distinctive features, some rheologic and physicochemical parameters of cheeses from four PDO producers, in distinct seasons and with different sensory scores, were monitored. The results suggested a high chemical diversity and variation according to the dairy, month and season, which corroborates the significant heterogeneity. However, a higher incidence of some compounds was found: a group of free amino acids (Glu, Ala, Leu, Val and Phe), lactic and acetic acids, some volatile fatty acids (e.g., iC4, iC5, C6 and C12) and esters (e.g., ethyl butanoate, decanoate and dodecanoate). Through the successive statistical analysis, 13 variables were selected as chemical markers of Serpa cheese specificity: C3, C4, iC5, C12, Tyr, Trp, Ile, 2-undecanone, ethyl isovalerate, moisture content on a fat-free basis, the nitrogen-fractions (maturation index and non-protein and total nitrogen ratio) and G’1 Hz. These sensory markers’ identification will be essential to guide the selection and development of an autochthonous starter culture to improve cheese quality and safety issues and maintain some of the Serpa authenticity.
Impact of LAB from Serpa PDO Cheese in Cheese Models
Publication . Araújo-Rodrigues, Helena; Martins, António P. L.; Tavaria, Freni K.; Dias, João; Santos, Maria Teresa; Alvarenga, Nuno; Pintado, Manuela E.; GeoBioTec - Geobiociências, Geoengenharias e Geotecnologias; MDPI - Multidisciplinary Digital Publishing Institute
Serpa is a protected designation of origin (PDO) cheese produced with raw ewes’ milk and coagulated with Cynara cardunculus. Legislation does not allow for milk pasteurization and starter culture inoculation. Although natural Serpa’s rich microbiota allows for the development of a unique organoleptic profile, it also suggests high heterogeneity. This raises issues in the final sensory and safety properties, leading to several losses in the sector. A possible solution to overcoming these issues is the development of an autochthonous starter culture. In the present work, some Serpa cheese Lactic acid bacteria (LAB)-isolated microorganisms, previously selected based on their safety, technological and protective performance, were tested in laboratory-scale cheeses. Their acidification, proteolysis (protein and peptide profile, nitrogen fractions, free amino acids (FAA)), and volatiles generation (volatile fatty acids (VFA) and esters) potential was investigated. Significant differences were found in all parameters analyzed, showing a considerable strain effect. Successive statistical analyses were performed to compare cheese models and Serpa PDO cheese. The strains L. plantarum PL1 and PL2 and the PL1 and L. paracasei PC mix were selected as the most promising, resulting in a closer lipolytic and proteolytic profile of Serpa PDO cheese. In future work, these inocula will be produced at a pilot scale and tested at the cheese level to validate their application.
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Fundação para a Ciência e a Tecnologia
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POR_NORTE
Número da atribuição
2020.05798.BD