BIOMIMETIC MEMBRANES SELECTIVE FOR ANTIBODY MOLECULES

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Bioinspired and Sustainable Chitosan Based Monoliths for Antibody Capture and Release

Publication . Barroso, Telma; Roque, Ana Cecília Afonso; Ricardo, Ana Isabel Nobre Martins Aguiar de Oliveira; DQ - Departamento de Química; CQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE); RSC - Royal Society of Chemistry

Chitosan-based monoliths activated by plasma technology induced the coupling of a robust biomimetic ligand, previously reported as an artificial Protein A, with high yields while minimizing the environmental impact of the procedure. Due to the high porosity, good mechanical and tunable physicochemical properties of the affinity chitosan-based monoliths, it is possible to achieve high binding capacities (150 ± 10 mg antibody per gram support), and to recover 90 ± 5% of the bound protein with 98% purity directly from cell-culture extracts. Therefore, the chitosan-based monoliths prepared by clean processes exhibit a remarkable performance for the one-step capture and recovery of pure antibodies or other biological molecules with biopharmaceutical relevance.

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Fundação para a Ciência e a Tecnologia

Número da atribuição

SFRH/BD/62475/2009