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Projeto de investigação
Green Solvents for Sustainable Production of Microalgae Products with Enhanced Properties
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Exploring the potential of deep eutectic systems for the preservation of the chemical profile and antibacterial potential of dill (Anethum graveolens L.) supercritical CO₂ extracts
Publication . Vladić, Jelena; Jerković, Igor; Pavić, Valentina; Cvetković, Dragoljub; Jokić, Stela; Aladić, Krunoslav; Pastor, Kristian; Duarte, Ana Rita C.; DQ - Departamento de Química; LAQV@REQUIMTE; Elsevier
This study evaluated the potential of deep eutectic systems (DESs) to enhance the chemical and microbiological stability, as well as the antibacterial activity of dill (Anethum graveolens L.) supercritical extracts, rich in carvone and limonene, over six months of storage. Various betaine-based DESs were tested for their ability to preserve the chemical integrity and enhance the antibacterial properties of dill extracts. Significant alterations in the headspace profile of extracts during storage indicated degradation reactions. The dill-Bet/LA extract system (dill extract in Bet/LA) proved most effective, significantly slowing degradation and enhancing antibacterial activity compared to the dill-control extract. Specifically, this system exhibited 20–40-fold greater efficacy against Gram-negative bacteria and 5–8-fold stronger activity against Gram-positive bacteria than the dill-control. Furthermore, the dill-Bet/LA system was also the most effective in preserving antimicrobial potency during storage, demonstrating its potential as a sustainable solution for extending the shelf life and bioactivity of dill supercritical extracts.
Corrigendum to “Innovative approach in sustainable agriculture
Publication . Ferreira, Alice; Vladić, Jelena; de Oliveira Corrêa, Diego; Butzke, Valéria Louzada Leal; Martins, Pedro L.; Ribeiro, Belina; Marques-dos-Santos, Cláudia; Acién, F. Gabriel; Gouveia, Luisa; Faculdade de Ciências e Tecnologia (FCT); Elsevier BV
The authors regret that the values of Table 6 are in the incorrect units. The corrected version of Table 6 is enclosed herewith. Furthermore, the author Jelena Vladić would like to add the DOI to her CEEC grant. Jelena Vladic would like to acknowledge her CEEC grant CEECIND/04909/2022 awarded by Fundação para a Ciência e Tecnologia at program CEEC IND5ed (https://doi.org/10.54499/2022.04909.CEECIND/CP1725/CT0014). The authors would like to apologise for any inconvenience caused.
Clean production of microalgae high-value lipid fraction
Publication . Vladic, Jelena; Radman, Sanja; Zizak, Zeljko; Besu, Irina; Jerkovic, Igor; Speranza, Lais Galileu; Hala, Ahmad Furqan; Kovacevic, Strahinja; Perreira, Hugo; Gouveia, Luisa; DQ - Departamento de Química; Faculdade de Ciências e Tecnologia (FCT); LAQV@REQUIMTE; Elsevier
Microalgae have emerged as a promising natural resource rich in bioactive compounds. Health-beneficial properties of microalgae, coupled with advantageous characteristics such as high biomass productivity, adaptability, robustness, and carbon dioxide mitigation, position them as a viable solution for global sustainable food production. This study explored clean and environmentally friendly processes to enhance the recovery of lipid bioactive fractions. Microwave (MW), enzymatic (ENZ), and ultrasound (US) pretreatments were applied to improve environmentally friendly extraction of lipid-based components using supercritical CO2. The effects of these pretreatments on extraction yield, chemical profiles, and cytotoxic properties of Chlorella vulgaris (Cv) and smooth C. vulgaris (sCv) extracts were investigated. Additionally, a Life Cycle Assessment (LCA) was conducted to evaluate environmental impacts. MW pretreatment achieved the highest yield increases, from 2.58 times (Cv) to 3.15 times (sCv). UHPLC-ESI-HRMS analysis revealed shifts in the distribution of pigments and derivatives caused by pretreatments, with ENZ extracts showing the most pronounced changes: pigments increased from 9.24% (control Cv) to 40.92% (Cv) and from 12.52% (control sCv) to 71.12% (sCv). Cv extracts exhibited greater activity against MDA-MB-453 cells, while sCv extracts from US pretreatment demonstrated the strongest effect on HeLa cells. The LCA indicated reduced environmental impacts of the pretreatment-enhanced processes up to 65% compared to the control. A scenario analysis was presented to show further possible impact reduction by recirculating the CO2 solvent and substituting the energy source. These findings provide valuable insights into sustainable and scalable green processes for recovering microalgal bioactive components.
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Entidade financiadora
Fundação para a Ciência e a Tecnologia
Programa de financiamento
CEEC IND5ed
Número da atribuição
2022.04909.CEECIND/CP1725/CT0014