A carregar...
Projeto de investigação
Sem título
Financiador
Autores
Publicações
Synthesis, characterization and biological activity of methotrexate-derived salts in lung cancer cells
Publication . Silva, Dário; Cordeiro, Sandra; Baptista, Pedro V.; Fernandes, Alexandra R.; Branco, Luis C.; Faculdade de Ciências e Tecnologia (FCT); UCIBIO - Applied Molecular Biosciences Unit; DCV - Departamento de Ciências da Vida; LAQV@REQUIMTE; RSC - Royal Society of Chemistry
Lung cancer is one of the deadliest types of cancer, and is a public health problem worldwide. Methotrexate (MTX), a class IV drug in the biopharmaceutical classification system, is a folate antagonist that has demonstrated efficacy in cancer treatment. A suitable combination of MTX as a di-anion and biocompatible counter ions allowed the modulation of their physicochemical properties. In this work, twelve MTX salts were prepared and characterized by 1H NMR, 13C NMR, and elemental analysis. The antiproliferative effects of MTX salts were studied in A459 and H1975 (lung cancer cell lines) with three promising results: [C12mim]2[MTX] (IC50 = 0.55 ± 0.25) > [C10-3-picoline]2[MTX] (IC50 = 0.94 ± 0.03) > [C10mim]2[MTX] (IC50 = 1.71 ± 0.23) in A549. These three MTX salts also demonstrated intrinsic apoptosis, avoiding necrosis and the formation of reactive oxygen species.
Unidades organizacionais
Descrição
Palavras-chave
Contribuidores
Financiadores
Entidade financiadora
Fundação para a Ciência e a Tecnologia
Programa de financiamento
OE
Número da atribuição
2020.09889.BD