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Elemental quantification of adults zebrafish from different vivaria using X-ray fluorescence
Publication . Leite, F. D.; Pessanha, S.; Barbosa, S.; Borges, A. C.; Pereira, N.; Domingues, I.; Silva, A. L. M.; LIBPhys-UNL; DF – Departamento de Física; GeoBioTec - Geobiociências, Geoengenharias e Geotecnologias; DCT - Departamento de Ciências da Terra; Elsevier
Zebrafish (Danio rerio) has been used as a model organism in several areas of research, including ecotoxicology, genetics and preclinical research. Investigating the elemental composition of adult zebrafish samples collected from different vivaria can provide valuable insights into various aspects of their physiology, influenced by intrinsic factors, such as gender, and extrinsic factors, including environmental conditions. In this study, adult zebrafish specimens from three different vivaria in Portugal (Biology Department of University of Aveiro (UA), Instituto Gulbenkian de Ciência (IGC) and Instituto de Medicina Molecular João Lobo Antunes (iMM), Lisbon) were analysed using X-ray fluorescence (XRF). The XRF system used was the M4 Tornado (Bruker, Germany) and quantitative analysis was performed using the inbuilt software through the Fundamental Parameters method considering a matrix of 7 % H, 23 % C, 7 % N and 60 % O. Twelve elements were detected and analysed, with Ca and P showing the highest concentrations across all vivaria. Fe, Zn, and Br exhibited significant gender differences in all vivaria, while Ca, Ni, and Cu showed no significant differences between genders or vivaria.
Shallow water fish display low phenotypic plasticity to ocean warming and extreme weather events regardless of previous thermal history
Publication . Missionário, Madalena; Almeida, Célia; Fernandes, Joana Filipa; Vinagre, Catarina; Madeira, Carolina; Madeira, Diana; DCV - Departamento de Ciências da Vida; Faculdade de Ciências e Tecnologia (FCT); UCIBIO - Applied Molecular Biosciences Unit; Elsevier Science B.V., Amsterdam.
Shallow water environments have long been recognized by scientists as sentinels for climate change. By analysing the impacts of ocean warming and marine heatwaves (MHW) in species from these areas, we can estimate their plasticity and hence vulnerability to thermal challenges. Pomatoschistus microps is a benthic intertidal fish species inhabiting coastal lagoons where temperature fluctuations are common. Here, we tested the effects of “Present” and “Future summer” scenarios (22 °C and 25 °C) and their respective heatwaves (27 °C and 30 °C) versus a “Control” scenario of 19 °C on warm (summer)- and cold (winter)- acclimatized fish. Then, we estimated phenotypic plasticity of critical thermal maximum (CTmax), oxygen consumption and cellular stress responses (CSR). Temperature seasonal variation and body weight (as proxy for body size) effects on fish thermal tolerance were also determined. Fish exposed to higher temperature treatments exhibited higher thermal tolerance, with this pattern being consistent for both warm- and cold-acclimatized fish. However, this difference was subtle (<4.6 %), suggesting a low capacity for acclimation. Nonetheless, warm-acclimatized fish (collected in summer) displayed significantly higher CTmax than cold-acclimatized fish (collected in winter), indicating that CTmax is influenced by seasonal thermal variation. Weight also represents a constraint factor for P. microps thermal tolerance, as heavier animals displayed lower CTmax. No alterations in O2 consumption, neither in CSR biomarkers were detected across temperature treatments, suggesting that fish were otherwise relatively insensitive to thermal fluctuations, independently of thermal history, within the thermal scenarios tested. Overall, the studied population of P. microps seems well adapted to temperature variations in their natural environment, exhibiting a large thermal safety margin (average of 11.02 °C).
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Fundação para a Ciência e a Tecnologia
Programa de financiamento
Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017/2018) - Financiamento Programático
Número da atribuição
UIDP/50017/2020