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Bacterial autolysins trim cell surface peptidoglycan to prevent detection by the drosophila innate immune system
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Orientador(es)
Resumo(s)
Bacteria have to avoid recognition by the host immune system in order to establish a successful infection. Peptidoglycan, the principal constituent of virtually all bacterial surfaces, is a specific molecular signature recognized by dedicated host receptors, present in animals and plants, which trigger an immune response. Here we report that autolysins from Gram-positive pathogenic bacteria, enzymes capable of hydrolyzing peptidoglycan, have a major role in concealing this inflammatory molecule from Drosophila peptidoglycan recognition proteins (PGRPs). We show that autolysins trim the outermost peptidoglycan fragments and that in their absence bacterial virulence is impaired, as PGRPs can directly recognize leftover peptidoglycan extending beyond the external layers of bacterial proteins and polysaccharides. The activity of autolysins is not restricted to the producer cells but can also alter the surface of neighboring bacteria, facilitating the survival of the entire population in the infected host.
Descrição
Fundacao para a Ciencia e Tecnologia PTDC/SAU-IMU/111806/2009 Sergio Raposo Filipe Wellcome Trust WT087680 Petros Ligoxygakis European Research Council ERC-2012-StG-310987 Mariana Gomes Pinho Fundacao para a Ciencia e Tecnologia PTDC/BIA-MIC/111817/2009 Sergio Raposo Filipe Fundacao para a Ciencia e Tecnologia PTDC/BIA-BCM/099152/2008 Mariana Gomes Pinho Fundacao para a Ciencia e Tecnologia PTDC/BIA-MIC/101375/2008 Rita Goncalves Sobral Fundacao para a Ciencia e Tecnologia PEst-OE/EQB/LA0004/2011 Mariana Gomes Pinho, Sergio Raposo Filipe Fundacao para a Ciencia e Tecnologia Fellowship SFRH/BD/28440/2006 Magda Luciana Atilano Fundacao para a Ciencia e Tecnologia Fellowship SFRH/BD/41119/2007 Pedro Matos Pereira Fundacao para a Ciencia e Tecnologia Fellowship SFRH/BD/78748/2011 Filipa Vaz Fundacao para a Ciencia e Tecnologia Fellowship SFRH/BD/77758/2011 Maria Joao Catalao Fundacao para a Ciencia e Tecnologia Fellowship SFRH/BD/23812/2005 Patricia Reed Fundacao para a Ciencia e Tecnologia Fellowship SFRH/BD/70162/2010 Ines Ramos Grilo The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Palavras-chave
Animals Drosophila Gram-Positive Bacteria Hydrolysis Immunity, Innate N-Acetylmuramoyl-L-alanine Amidase Peptidoglycan Virulence General Biochemistry,Genetics and Molecular Biology General Immunology and Microbiology General Medicine General Neuroscience