Please use this identifier to cite or link to this item: http://hdl.handle.net/10362/127577
Title: The Off-Targets of Clustered Regularly Interspaced Short Palindromic Repeats Gene Editing
Author: Vicente, Manuel M.
Chaves-Ferreira, Miguel
Jorge, João M.P.
Proença, João T.
Barreto, Vasco M.
Keywords: Cas9 variants
CRISPR/Cas9
DNA repair
gene editing
off-targets
Developmental Biology
Cell Biology
Issue Date: 17-Sep-2021
Abstract: The repurposing of the CRISPR/Cas bacterial defense system against bacteriophages as simple and flexible molecular tools has revolutionized the field of gene editing. These tools are now widely used in basic research and clinical trials involving human somatic cells. However, a global moratorium on all clinical uses of human germline editing has been proposed because the technology still lacks the required efficacy and safety. Here we focus on the approaches developed since 2013 to decrease the frequency of unwanted mutations (the off-targets) during CRISPR-based gene editing.
Description: Funding: The work of VMB was funded by iNOVA4Health – UIDB/Multi/04462/2020 and UIDP/Multi/04462/2020, a program financially supported by Fundação para a Ciência e Tecnologia (FCT)/Ministério da Educação e Ciência through national funds, and the FCT grant PTDC/BEX-BCM/5900/2014.
Peer review: yes
URI: http://hdl.handle.net/10362/127577
DOI: https://doi.org/10.3389/fcell.2021.718466
ISSN: 2296-634X
Appears in Collections:NMS: CEDOC - Artigos em revista internacional com arbitragem científica

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