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Insights into the role of almond CBF transcription factors in the environmental control of cold acclimation and dormancy break
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Cold is an important environmental factor that may influence plant
survival and productivity. It also plays a crucial role in the seasonal
development of perennial plants by regulating the extent of (winter)
dormancy and that of vegetative and reproductive growth periods. This
aspect is particularly relevant in fruit trees, since it may directly impact fruit
production. The Prunus genus includes economically relevant species, and
the almond (Prunus dulcis Mill.) stands out for its high range of genetic
variability, thus being an interesting model to study specific traits of
agricultural interest. Considering that low temperature signalling pathways
are still poorly understood in Prunus spp., this study aimed to identify and
elucidate, in almonds, the function of members of the C-repeat Binding
Factor (CBF) family of transcription factors (TFs) which are known to be
relevant in the response to cold. Therefore, two CBF genes (PrdCBF1 and -
2) and their corresponding regulatory regions were cloned and sequenced
in this species. Southern-blot analysis and further comparisons to the
recently available peach genome revealed that the almond CBF family is
composed of at least five members, located in close proximity in
chromosome 5. In addition, a mapping analysis suggested an association
between PrdCBFs and PrdDehydrin1 (PrdDHN1, a putative CBF target
gene, involved in cold acclimation) to recently reported QTLs controlling
chilling requirements and blooming time. However, further analyses are
necessary to confirm this association.(...)
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Dissertation presented to obtain the Ph.D degree in Biology
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Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica