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http://hdl.handle.net/10362/188928
Título: | Cotyledon opening during seedling deetiolation is determined by ABA-mediated splicing regulation |
Autor: | Martín, Guiomar Confraria, Ana Zapata, Irene Larran, Alvaro Santiago Qüesta, Julia Irene Duque, Paula |
Palavras-chave: | Abscisic Acid Alternative Splicing Photomorphogenesis Seedling Deetiolation SR Proteins Biochemistry Molecular Biology Genetics |
Data: | 18-Jun-2025 |
Resumo: | During seedling deetiolation, plants adjust their development to expose photosynthetic tissues to sunlight, enabling the transition from heterotrophic to autotrophic growth. While various plant hormones are known to influence this process, the role of abscisic acid (ABA) remains unclear. Here, we reveal that ABA plays a major role in controlling the dynamics of cotyledon aperture during seedling deetiolation. In the dark, ABA accumulates in the cotyledons to effectively repress their opening. However, light exposure reverses this effect, allowing the cotyledons to open. Our findings indicate that ABA-mediated regulation of cotyledon dynamics is accompanied by genome-wide rearrangements in both transcriptional and splicing patterns. We demonstrate that ABA-dependent adjustments of cotyledon and splicing dynamics in response to light depend on the positive role of two splicing factors, RS40 and RS41. Moreover, we identify transcriptional and posttranscriptional mechanisms that control the activity of these proteins. Altogether, this work sheds light on the interplay between light and ABA, highlighting cotyledon opening as a new developmental outcome, and identifying alternative splicing as the underlying layer of gene regulation. |
Descrição: | Funding Information: The authors thank Andreas Wachter, Pedro Rodr\u00EDguez, and Elena Baena-Gonz\u00E1lez for kindly providing the rs40-2, rs41-1, pyl1458, snrk2-2.3.6 and abi1-1 mutants, Nil Veciana for technical assistance with the Leica DM6 epifluorescent microscope, and Vera Nunes for excellent plant care. This work was funded by Funda\u00E7\u00E3o para a Ci\u00EAncia e a Tecnologia (FCT) through grants PTDC/BIA-BID/30608/2017, PTDC/ASP-PLA/2550/2021 ( https://doi.org/10.54499/PTDC/ASP-PLA/2550/2021 ), CEECIND/02655/2017/CP1428/CT0003 ( https://doi.org/10.54499/CEECIND/02655/2017/CP1428/CT0003 ), 2023.06360.CEECIND/CP2836/CT0009 (10.54499/2023.06360.CEECIND/CP2836/CT0009), UIDB/04551/2020 ( https://doi.org/10.54499/UIDB/04551/2020 ) and UIDP/04551/2020 ( https://doi.org/10.54499/UIDP/04551/2020 ) as well as by the Spanish Ministry of Science and Innovation through grant PID2021-125223NA-I00 (MCIN/AEI/10.13039/501100011033/FEDER). GM was supported by a Ram\u00F3n y Cajal fellow (RYC2020-030160_I; MCIN/AEI/10.13039/501100011033/FSE) from the Spanish Ministry of Science and Innovation, an EMBO Long-Term Fellowship (ALTF 1576-2016) and a Marie Sk\u0142odowska-Curie Individual Postdoctoral Fellowship (EU project 750469). JIQ was a Ram\u00F3n y Cajal fellow (RYC2021-032539-I). ASL received funding from the AGenT Programme, a European Union\u2019s Horizon 2020 research and innovation Marie Sk\u0142odowska-Curie (MSCA) COFUND program under grant agreement no. 945043. Publisher Copyright: © The Author(s) 2025. |
Peer review: | yes |
URI: | http://hdl.handle.net/10362/188928 |
DOI: | https://doi.org/10.1038/s44319-025-00495-5 |
ISSN: | 1469-221X |
Aparece nas colecções: | Home collection (ITQB) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Cotyledon_opening_during_seedling_deetiolation_is.pdf | 1,83 MB | Adobe PDF | Ver/Abrir |
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