Natural Colorants for a Bio-Based Economy

Cleveland, Imogen; Beeby, Andrew; Vieira, Márcia; Pina, Fernando; Branco, Paula S.; Nabais, Paula; Melo, Maria J.

http://hdl.handle.net/10362/187215

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Natural colorants, with their sustainable origins, offer a promising alternative for various applications. Advanced studies have unveiled the remarkable properties, resilience, and durability of these ancient dyes, which our ancestors developed through sustainable material processing. This serves as a testament to the potential of sustainable solutions in our field. As part of our research, we prepared three medieval temperas using gum arabic, parchment glue, and casein glue. These tempera were explicitly designed to protect the purples obtained from Chrozophora tinctoria extracts. A comprehensive multi-analytical approach guides our research on natural colorants. Central to this approach is the use of molecular fluorescence by microspectrofluorimetry, a key tool in our study. By analyzing the emission and excitation spectra in the visible range, we can identify specific formulations. This method is further supported by fingerprinting techniques, including Fourier Transform Infrared Spectroscopy (FTIR) and High-Performance Liquid Chromatography with Diode Array Detection (HPLC-DAD). These are further complemented by Fiber Optics Reflectance Spectroscopy (FORS) and colorimetry. Building on our understanding of orcein purples, we have extended our research to purples derived from Chrozophora tinctoria extracts. Our findings reveal the unique properties of Chrozophora tinctoria, which can be accurately distinguished from orcein purples, highlighting the distinctiveness of each.

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Funding Information: This work received financial support from FCT/MCTES through national funds; LA/P/0008/2023 DOI 10.54499/LA/P/0008/2020, UIDP/50006/2020 DOI 10.54499/UIDP/50006/2023 and UIDB/50006/2023 DOI 10.54499/UIDB/50006/2023; 2021.01344.CEECIND/CP1657/CT0028. Publisher Copyright: © 2025 by the authors.

Palavras-chave

Chrozophora tinctoria Cultural heritage Microspectrofluorimetry Organic dyes Analytical Chemistry Chemistry (miscellaneous) Molecular Medicine Pharmaceutical Science Drug Discovery Physical and Theoretical Chemistry Organic Chemistry