Utilize este identificador para referenciar este registo: http://hdl.handle.net/10362/177087
Título: Optical fiber immunosensors based on surface plasmon resonance for the detection of Escherichia coli
Autor: Oliveira, Sandro C.
Soares, Simone
Rodrigues, Andreia C. M.
Gonçalves, Bárbara V.
Soares, Amadeu M. V. M.
Santos, Nuno
Kumar, Santosh
Almeida, Pedro
Marques, Carlos
Palavras-chave: Atomic and Molecular Physics, and Optics
SDG 3 - Good Health and Well-being
SDG 6 - Clean Water and Sanitation
SDG 14 - Life Below Water
Data: 11-Mar-2024
Citação: Oliveira, S. C., Soares, S., Rodrigues, A. C. M., Gonçalves, B. V., Soares, A. M. V. M., Santos, N., Kumar, S., Almeida, P., & Marques, C. (2024). Optical fiber immunosensors based on surface plasmon resonance for the detection of Escherichia coli. Optics Express, 32(6), 10077-10092. https://doi.org/10.1364/OE.518723
Resumo: Every year, millions of people suffer some form of illness associated with the consumption of contaminated food. Escherichia coli (E. coli), found in the intestines of humans and other animals, is commonly associated with various diseases, due to the existence of pathogenic strains. Strict monitoring of food products for human consumption is essential to ensure public health, but traditional cell culture-based methods are associated with long waiting times and high costs. New approaches must be developed to achieve cheap, fast, and on-site monitoring. Thus, in this work, we developed optical fiber sensors based on surface plasmon resonance. Gold and cysteamine-coated fibers were functionalized with anti-E. coli antibody and tested using E. coli suspensions with concentrations ranging from 1 cell/mL to 105 cells/mL. An average logarithmic sensitivity of 0.21 ± 0.01 nm/log(cells/mL) was obtained for three independent assays. An additional assay revealed that including molybdenum disulfide resulted in an increase of approximately 50% in sensitivity. Specificity and selectivity were also evaluated, and the sensors were used to analyze contaminated water samples, which verified their promising applicability in the aquaculture field.
Descrição: This work is part of the project DepurD (MAR-01.03.01-FEAMP-0046), supported by Portugal and the European Union through MAR2020, Portugal2020, and FEAMP. Publisher Copyright: © 2024 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.
Peer review: yes
URI: http://hdl.handle.net/10362/177087
DOI: https://doi.org/10.1364/OE.518723
ISSN: 1094-4087
Aparece nas colecções:FCT: CENIMAT - Artigos em revista internacional com arbitragem científica

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