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Selenium Bio-Nanocomposite Based on Alteromonas macleodii Mo169 Exopolysaccharide
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Orientador(es)
Resumo(s)
In this study, the novel exopolysaccharide (EPS) produced by the marine bacterium Alteromonas macleodii Mo 169 was used as a stabilizer and capping agent in the preparation of selenium nanoparticles (SeNPs). The synthesized nanoparticles were well dispersed and spherical with an average particle size of 32 nm. The cytotoxicity of the EPS and the EPS/SeNPs bio-nanocomposite was investigated on human keratinocyte (HaCaT) and fibroblast (CCD-1079Sk) cell lines. No cytotoxicity was found for the EPS alone for concentrations up to 1 g L−1. A cytotoxic effect was only noticed for the bio-nanocomposite at the highest concentrations tested (0.5 and 1 g L−1). In vitro experiments demonstrated that non-cytotoxic concentrations of the EPS/SeNPs bio-nanocomposite had a significant cellular antioxidant effect on the HaCaT cell line by reducing ROS levels up to 33.8%. These findings demonstrated that the A. macleodii Mo 169 EPS can be efficiently used as a stabilizer and surface coating to produce a SeNP-based bio-nanocomposite with improved antioxidant activity.
Descrição
Funding Information:
This work was financed by national funds from FCT—Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project LA/P/0140/202019 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. Funding from the INTERFACE Programme, through the Innovation, Technology and Circular Economy Fund (FITEC), is gratefully acknowledged. ATS also acknowledges FCT/MCTES for the Individual Grant CEEC-IND/04801/2017.
Publisher Copyright:
© 2023 by the authors.
Palavras-chave
Alteromonas macleodii Mo 169 bioactive material blue biotechnology exopolysaccharide (EPS) selenium nanoparticles (SeNP) Bioengineering SDG 14 - Life Below Water