High-resolution shotgun proteomics reveals that increased air [CO2] amplifies the acclimation response of coffea species to drought regarding antioxidative, energy, sugar, and lipid dynamics

Marques, Isabel; Rodrigues, Ana P.; Gouveia, Duarte; Lidon, Fernando C.; Martins, Sónia; Semedo, Magda C.; Gaillard, Jean Charles; Pais, Isabel P.; Semedo, José N.; Scotti-Campos, Paula; Reboredo, Fernando H.; Partelli, Fábio L.; DaMatta, Fábio M.; Armengaud, Jean; Ribeiro-Barros, Ana I.; Ramalho, José C.

http://hdl.handle.net/10362/145852

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High_resolution_shotgun_proteomics_reveals_that_increased_air_CO2_amplifies_the_acclimation_response_of_coffea_species_to_drought_regarding_antioxidative_energy_sugar_and_lipid_dynamics.pdf		1.91 MB	Adobe PDF	Ver/Abrir

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Autores

Gaillard, Jean Charles

Pais, Isabel P.

Semedo, José N.

Scotti-Campos, Paula

Resumo(s)

As drought threatens crop productivity it is crucial to characterize the defense mechanisms against water deficit and unveil their interaction with the expected rise in the air [CO2]. For that, plants of Coffea canephora cv. Conilon Clone 153 (CL153) and C. arabica cv. Icatu grown under 380 (aCO2) or 700 μL L−1 (eCO2) were exposed to moderate (MWD) and severe (SWD) water deficits. Responses were characterized through the activity and/or abundance of a selected set of proteins associated with antioxidative (e.g., Violaxanthin de-epoxidase, Superoxide dismutase, Ascorbate peroxidases, Monodehydroascorbate reductase), energy/sugar (e.g., Ferredoxin-NADP reductase, NADP-dependent glyceraldehyde-3-phosphate dehydrogenase, sucrose synthase, mannose-6-phosphate isomerase, Enolase), and lipid (Lineolate 13S-lipoxygenase) processes, as well as with other antioxidative (ascorbate) and protective (HSP70) molecules. MWD caused small changes in both genotypes regardless of [CO2] level while under the single imposition to SWD, only Icatu showed a global reinforcement of most studied proteins supporting its tolerance to drought. eCO2 alone did not promote remarkable changes but strengthened a robust multi-response under SWD, even supporting the reversion of impacts already observed by CL153 at aCO2. In the context of climate changes where water constraints and [CO2] levels are expected to increase, these results highlight why eCO2 might have an important role in improving drought tolerance in Coffea species.

Descrição

This work received funding support from the European Union's Horizon 2020 research and innovation program (grant agreement No 727934 , project BreedCAFS), ( CEF ), - Individual Call (CEEC Individual) - 2021.01107.CEECIND/CP1689/CT0001 (IM) . Fellowships from the Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil (CNPq) , (to F.M. DaMatta and F.L. Partelli), and Fundação de Amparo à Pesquisa do Estado de Minas Gerais, Brazil (FAPEMIG, projects CRA-RED-00053-16 and APQ 01512-18 , to F.M. DaMatta) are also greatly acknowledged. Publisher Copyright: © 2022 Elsevier GmbH

Palavras-chave

Acclimation Antioxidative response Climate change Coffee tree Elevated air [CO2] HSP70 Proteomic analysis Physiology Agronomy and Crop Science Plant Science SDG 13 - Climate Action