Energy dispersive X-ray fluorescence quantitative analysis of biological samples with the external standard method

Abstract

Trace elements are present in minute amounts in the human body but contribute to its proper functioning, by participating in several biological processes. Imbalance of the concentrations of these elements can lead to the development of pathologies, including cancer. As such, the determination of trace element content in tumour tissues and its comparison with normal ones may be helpful for a better understanding of carcinogenesis. In this work, we address the collection and preparation of biological samples for Energy Dispersive X-Ray Fluorescence (EDXRF) analysis, and present a model for the quantification of trace elements, based on the external standard method of quantification. The model was used for the quantification of iron, copper, and zinc in a set of paired samples (normal and tumour tissues). The obtained results show the validity of the method and variations of the elemental concentrations in the different tissues.