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Investigation of the genotoxicity of digested titanium dioxide nanomaterials in human intestinal cells

2022-03Artigo científico Acesso aberto
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dc.contributor.authorVieira, Adriana
dc.contributor.authorVital, Nádia
dc.contributor.authorRolo, Dora
dc.contributor.authorRoque, Rossana
dc.contributor.authorGonçalves, Lídia M.
dc.contributor.authorBettencourt, Ana
dc.contributor.authorSilva, Maria João
dc.contributor.authorLouro, Henriqueta
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.institutionCentre for Toxicogenomics and Human Health (ToxOmics)
dc.contributor.pblElsevier Science B.V., Amsterdam.
dc.date.accessioned2022-02-23T23:17:37Z
dc.date.available2022-02-23T23:17:37Z
dc.date.issued2022-03
dc.descriptionFunding: This work was funded by national funds through the FCT - Foundation for Science and Technology, I.P., under the project PTDC/SAUPUB/29481/2017. Research co-funded by UIDB/00009/2020; UIDP/ 00009/2020 (Centre for Toxicogenomics and Human Health – ToxOmics, FCT- Foundation for Science and Technology). NV holds a FCT PhD Scholarship grant (2020.07168.BD). iMed.ULisboa (UIDB/04138/ 2020 and UIDP/04138/2020) principal investigator grants CEECIND/ 03143/2017 (L. M. Gonçalves). The authors thank all the support from the colleagues Paula Alvito, Carla Martins and Ricardo Assunç˜ ao (Food Safety Department, INSA, Lisbon, Portugal) as well from all INGESTnano team members.
dc.description.abstractThe widespread use of titanium dioxide nanomaterials (TiO2 NMs) in food and consumer products such as toothpaste or food contact materials, suggests the relevance of human oral exposure to these nanomaterials (NMs) and raises the possibility of adverse effects in the gastrointestinal tract (GIT). We previously showed that the in vitro digestion of TiO2 NMs may increase their toxicity in intestinal cells. In this work, we analyzed the genotoxicity and the intracellular reactive oxygen species induction by physiologically relevant concentrations of three different TiO2 NMs (NM-102, NM-103 and NM-105) in Caco-2 and HT29-MTX-E12 intestinal cells, while considering the potential influence of the digestion process in the NMs’ physiochemical characteristics. The results evidenced a DNA-damaging effect dependent on the NM, more relevant for the rutile/anatase NM-105, possibly due to its lower hydrodynamic size in the cells medium. In addition, the results of the micronucleus assay suggest effects on chromosomal integrity, an indicator of cancer risk, in the HT29-MTX-E12 cells, for all the tested TiO2 NMs, especially after the in vitro digestion. This work supports the evidence for concerns on the use of TiO2 NMs as a food additive, recently reported by EFSA, and for their use in applications in consumer products that may drive human exposure through ingestion.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent7921132
dc.identifier.doi10.1016/j.fct.2022.112841
dc.identifier.issn0278-6915
dc.identifier.otherPURE: 41736201
dc.identifier.otherPURE UUID: 98252a39-3935-49cf-a8d5-c732bdd3c637
dc.identifier.otherScopus: 85123757548
dc.identifier.otherWOS: 000750804400001
dc.identifier.otherPubMed: 35093430
dc.identifier.urihttp://hdl.handle.net/10362/133484
dc.identifier.urlhttps://www.scopus.com/pages/publications/85123757548
dc.language.isoeng
dc.peerreviewedyes
dc.subjectGenotoxicity
dc.subjectIn vitro simulated digestion
dc.subjectIntestinal epithelial cells
dc.subjectNanomaterials
dc.subjectTitanium Dioxide
dc.subjectFood Science
dc.subjectToxicology
dc.subjectSDG 3 - Good Health and Well-being
dc.titleInvestigation of the genotoxicity of digested titanium dioxide nanomaterials in human intestinal cellsen
dc.typejournal article
degois.publication.titleFood and Chemical Toxicology
degois.publication.volume161
dspace.entity.typePublication
rcaap.rightsopenAccess

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