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PRELIMINARY RESULTS OF PHAGE DISPLAY METHOD TO PRODUCE RECOMBINANT ANTI-Schistosoma mansoni ANTIBODIES
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Orientador(es)
Resumo(s)
Schistosomiasis is a parasitic disease caused by trematodes of the genus Schistosoma. An accurate diagnosis of schistosomiasis is essential for treatment and disease control programs. However, diagnostic techniques may have some sensitivity limitations, especially in mild infections with low egg elimination rates. Therefore, the present study aims to select recombinant antibodies against S. mansoni antigens to be applied in the future serodiagnosis of schistosomiasis. Adult parasites of S. mansoni were extracted by perfusion of the liver of infected mice. The recombinant antibody methodology was based on phage display, involving three rounds of selection by biopanning. The visualization of the antigen-antibody binding was performed through an indirect ELISA test. The first two selection rounds of biopanning showed positive values for the presence of phages, but the third cycle did not generate a significant amount of phage particles. For the indirect ELISA test, 15 male and 15 female clones were selected. It was observed that most of the antibodies reacted similarly to both male and female antigens. This work has provided valuable information that could be useful for developing diagnostic technologies for Schistosomiasis.
Descrição
Funding Information: We are very thankful to Eleuza Machado for helping us by providing access to the laboratory facilities at the Anhanguera Educacional in Bras\u00EDlia/ Brazil, Luciana Galv\u00E3o for making available help at the Catholic University of Bras\u00EDlia, Silvana Belo for revising the text and Funda\u00E7\u00E3o para a Ci\u00EAncia e a Tecnologia for funds to GHTM - UID/04413/2020 and LA-REAL \u2013 LA/P/0117/2020. Publisher Copyright: © 2024 Brazilian Society of Parasitology. All rights reserved.
Palavras-chave
biopanning bioprospecting recombinant antibodies Schistosomiasis General Immunology and Microbiology Public Health, Environmental and Occupational Health Infectious Diseases SDG 3 - Good Health and Well-being